List of Articles Aquaporin9

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  1 - immunohistochemical evaluation in testicular tissue of male rats following PM2.5 particle inhalation
  elnaz noshadirad kazem parivar Saeed Motesaddi Zarandi Pejman Mortazavi batool Gorbani yekta
  Background & Aim: Exposure to suspended particles (PM2.5) and gaseous air pollution poses a serious threat to spermatogenesis in men. However, the specific molecular mechanism behind this remains largely unknown. This study aims to investigate the impact of PM2.5 and ga More

  Background & Aim: Exposure to suspended particles (PM2.5) and gaseous air pollution poses a serious threat to spermatogenesis in men. However, the specific molecular mechanism behind this remains largely unknown. This study aims to investigate the impact of PM2.5 and gas pollution on various aspects including the oxidant/antioxidant system, oxidative stress and also the changes in the expression level of aquaporin protein through immunohistochemistry and western blotting in testicular tissue were investigated. Materials & Methods: A total of 36 male Wistar rats were randomly divided into three groups: a control group exposed to standard air conditions, a group exposed to gas pollutants alone (Gas), and a group exposed to both gas pollutants and PM2.5 (Gas+PM2.5). All groups were subjected to pollutant exposure for three months, four days a week, and five hours a day. Results: The findings revealed that co-exposure to gaseous pollutants and PM2.5 led to increased concentration of Malondialdehyde (MDA), as well as decreased in aquaporin 9 protein in immunohistochemistry and western blot, compared to the control group. Conclusion: Therefore, exposure to PM2.5 and gaseous pollutants probably provokes oxidative stress in the testis, which leads to the reduction of aquaporin 9 protein through the activation of signaling pathways. Thus, PM2.5 pollution appears to play a crucial role in infertility by disrupting spermatogenesis. Manuscript profile
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  2 - The effect of PM2/5 exposure on Hormonal changes, sperm DNA fragmentation and Aquaporin9 gene methylation in Wistar testis tissue
  elnaz noshadirad K parivar saeed Motesaddi Zarandi B Gorbani Yekta
  Air pollution in different cities of the world, has become a one of the most important environmental problems, which the effect of its on human health, especially fertility, has been considered. This study aims to investigate the impact of PM2/5 and gas pollution on va More

  Air pollution in different cities of the world, has become a one of the most important environmental problems, which the effect of its on human health, especially fertility, has been considered. This study aims to investigate the impact of PM2/5 and gas pollution on various aspects including the, sex hormone changes, sperm DNA fragmentation and also (MS-HRM) assay of aquaporin 9 gene in testis tissue. After sampling and evaluating the concentration of PM2/5 24 Wistar rats (weight 170 to 200 g ) were used. The animals were randomly divided into three equal groups including the exposure group 1 (received PM2/5 and gaseous pollutants), exposure group 2 (received gaseous pollutants), and the control group (received the air filtered). After 6 months the animals were investigated for hormonal changes such as FSH, testosterone as well as sperm DNA fragmentation and also methylation-sensitive high-resolution melting (MS-HRM) assay was performed. The data obtained showed that the serum level of FSH and testosterone are significantly increased and decreased in the exposure group 1 compared to the control group, respectively. The exposure group 2 also significantly showed lower the testosterone level than that of the control group. A significant difference was also seen in both exposure groups 1 and 2 with the control group respected to sperm DNA fragmentation. MS-HRM assays detected only hypermethylation of Exposure 1 in comparison with control group in a period of six months. Therefore, exposure to PM2/5 and gas pollutants leads to interference in the secretion of sex hormones and has a detrimental effect on sperm DNA fragmentation, resulting in disruption of male reproductive function, which is crucial in inducing infertility in males. These findings may help in identifying therapeutic targets for reducing human infertility disorders caused by exposure to PM2/5. Manuscript profile