Phylogenetic analysis of Afshari sheep population based on HVR and cytB regions of mitochondrial DNA
Subject Areas : animal development
1 - Department of Animal Science, Faculty of Agriculture, Varamin pishva Branch, Islamic Azad University, Varamin, Iran
Keywords: Afshari sheep, DNA sequencing, phylogeny, mtDNA, HVR1 and CytB regions,
Abstract :
Background and objectives: Sheep as a domestic animal have played a key role in food resources of Iranian plateau people and this area is known as the origin of this spice. Among the genetic markers mtDNA sequencing is one of the most useful and common methods employed for inferring phylogenetic relationship among closely related species and population and conservation of species. Materials and Methods: For this study 40 blood samples from unrelated Afshari sheep were collected. The DNA was extracted and the HVR1 and cytB regions of mtDNA were amplified with specific primers using PCR. Results: The HVR1 and cytB of mtDNA of Afshari breed were sequenced, alingned and compared with other breeds from all over the world. Results showed 3 and 5 haplotype respectively. In addition both loci were high polymorphicConclusion: Sequencing of mitochondrial genome can showed genetic variation and genetic relationship between oviane in the world. For this reason it is powerful and applicable tool to determine the level of genetic diversity.
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