The study of the association of two variants of MLH3(rs175080) andTEX11(rs6525433) in Iranian infertile men

Subject Areas : genetics

Hanieh Sezavar ¹ , Zahra Noormohammadi ² , Masoud Sheidai ³

1 - Science and Research Branch, Islamic Azad University, Poonak, Tehran
2 - Science and Research Branch, Islamic Azad university, Poonak, Tehran
3 - Science And Research Branch, Islamic Azad University, Poonak, Tehran

Received: 2020-12-05 Accepted : 2021-01-25 Published : 2020-02-20

Keywords: polymorphism, azoospermia, Male infertility, MLH3, TEX11 gene,

Abstract :

Infertility is one of the most common problems in human societies which estimated between 1 in 6 couples worldwide. It is a complex clinical condition caused by the interaction of genetic and environmental factors. The TEX11 and MLH3 gene plays a key role in the mechanism of DNA repair and crossing over. Methods: In this study, the association of two SNPs of MLH3 (rs175080) and TEX11 (rs6525433) in Iranian infertile men was investigated. One hundred twenty infertile men with azoospermia and 115 healthy individuals were collected as a control. These two polymorphisms were investigated by using tetra-amplification refractory mutation system ((PCR), TETRA - ARMS – PCR), PCR – RFLP, and sequencing were used. Findings and Discussion: The frequency of GG and GA genotypes in MLH3 gene was 30.9% and 69.1% in azoospermia males and 31.3% and 68.7% in the control group (P = 0.619), respectively. The result showed that rs175080 polymorphism in the MLH3 gene and rs6525433 polymorphism in the TEX11 gene were not associated with the risk of Azoospermia.