Determining of non-toxic dose of Fe3O4 magnetic nanoparticles on amniotic membrane stem cells
Subject Areas : cellular bilology
neda mousaviniri
1
,
maryam naseroleslami
2
1 - Department of Medical Biotechnology, Faculty of Advanced Sciences and technology, Tehran Medical Sciences,Islamic azad university, Tehran, Iran
2 - Department of Cellular and Molecular Biology, Faculty of Advanced Sciences and technology, Tehran Medical Sciences,Islamic azad university, Tehran, Iran
Keywords: reactive oxygen species, Rhodamine 123, amniotic membrane stem cells,
Abstract :
Superparamagnetic iron oxide nanoparticles (SPIONs) are an Important advancement in the field of nanotechnology. They expand the possibilities of noninvasive analysis for tracking of cells and have many useful properties, making them potential candidates for numerous applications in medicine. However, the possibility of toxicity in cells is reported by these nanoparticles. The goal of this study was to find a concentration of SPIONs that cant induce intracellular levels of Reactive Oxygen Species. For this purpose for the first time amniotic membrane stem cells were incubated with different concentrations of SPIONs coated with polyethylene glycol (PEG). Then intracellular levels of Reactive Oxygen Species was measured By probe Rhodamine 123 . Our results demonstrate that there was no significant generators of Reactive Oxygen Species in cells in concentration range of 0-100 μg/mL of spions. However, at concentrations higher than 100 μg / ml, the production of Reactive Oxygen Species increased. According to the results nanoparticles used in this study at concentrations ≤100 μg/mL are suitable for tracking of this cells.
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