Determination of the diagnostic value of milk lactate dehydrogenase and alkaline phosphatase in bovine subclinical mastitis caused by streptococcus uberis
Subject Areas : Journal of Large Animal Clinical Science Research(JLACSR)
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Keywords: lactate dehydrogenase, Alkaline phosphatase, subclinical mastitis, Streptococcus uberis,
Abstract :
Mastitis is the most common and damaging problem of dairy herds all over the world. On the other hand, environmental streptococci such as Streptococcus uberis have become one of the most important causes of mastitis in dairy cows. To identify infected animals, new markers are needed that are cost-effective and highly accurate. For this purpose, 145 clinically healthy Holstein cows were randomly selected from 7 industrial dairy farms in Tehran and Mazandaran provinces. Milk samples were taken from healthy and subclinical mastitis cows. Blood serum and defatted milk were used to measure the activities of lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) using commercial kits. From a total of 20 cartiers suffering from subclinical mastitis caused by Streptococcus eubris, 12 diagonal cartiers were found to be healthy and were considered as control cartiers of the same cow. Sensitivity, specificity and cutoff points of each test were determined by ROC curve. Milk LDH at the cutoff point of 133.5 u/l had 85% sensitivity, 91.7% specificity, and high clinical accuracy (>0.9) in the diagnosis of Streptococcus uberis-infected cartiers. Also, the milk ALP at the cutoff point of 386.5 u/l had 70% sensitivity, 75% specificity and moderate clinical accuracy (0.813) in diagnosing Cartiers with subclinical mastitis caused by Streptococcus uberis. The results of this research showed that the measurement of milk LDH and ALP activity has a high diagnostic value in the diagnosis of subclinical mastitis caused by Streptococcus uberis.
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