A Survey on Different Methods of Diagnosing Hydatid Cyst in Human
Subject Areas : Journal of Large Animal Clinical Science Research(JLACSR)
Keywords: hydatid cyst, Diagnostic methods, Serologic,
Abstract :
Cystic echinococcosis (CE) caused by infection with the larval stage of Echinococcus granulosus is of public health importance in Iran and other countries. Diagnosis relies primarily on the interpretation of results taken from both radiological imaging techniques and immunodiagnosis. However, clinical symptoms do not emerge until the larval cyst structure of the parasite has reached a certain size. During this time, immunodiagnosis has also proved effective and can conveniently be used to diagnose the disease in asymptomatic high risk populations. Various serological tests have been used to detect antibodies or antigens. Indirect haemagglutination test (IHA), indirect fluorescent antibody (IFA), Counter immuno-electrophorese (CIEP), Latex agglutination test (LAT), Western blotting (WB), Enzyme linked immunosorbent assay (ELISA), Dot-ELISA and … are common in human medicine. But, source of antigens and used methods have affects on diagnosing the disease. In addition, various strains of hydatid cyst often differ in biological characteristics which can influence the epidemiology of hydatidosis and measures taken to diagnose, treat and control the disease. The aim of the present study is to apply and evaluate the different methods in the diagnosis of hydatid cyst infection.
1- حسینی، س.ح.، مشگی، ب. (1389): انگل‌شناسی دامپزشکی (کرم‌های گرد و پهن) انتشارات دانشگاه تهران.
2- دلیمی اصل، عبدالحسین (1378): ارزیابی آزمون هماگلوتیناسیون غیرمستقیم در تشخیص هیداتیدوزیس گوسفندی. مجله پژوهش و سازندگی، صفحه 40.
3- فکور، ش. مشگی، ب. (1388): شناسایی آنتی ژن اختصاصی کیست هیداتیک با روش وسترن بلاتینگ به منظور کاربرد تشخیصی.مجله دانشگاه علوم پزشکی کردستان .سال چهاردهم. شماره دوم .تابستان1388
4- Burgu, A. (2000): Analysis of fluids of hydatid cyst from sheep by SDS-PAGE, and determination of specific antigens in protein structure by western blotting. Turkish Journal Veterinary Animal Science. 24: 493-500.
5- Craig, P. S. (1997): Immunodiagnosis of Echinococcus granulosus and a comparison of techniques for diagnosis of canine echinococcosis. In: Andersen, F.L., Ouhelli, H., Kachani, M. (eds) Compendium on Cystic Echinococcosis in Africa and Middle Eastern countries with special reference to Morocco. Provo, Utah: Brigham Young University. 85 – 118.
6- Doiz, O., Benito, R., Sbihi, Y., Osuna, A., Clavel, A., Gomez-Lus, R. (2001): Western blot applied to the diagnosis and post-treatment monitoring of human hydatidosis. Diagnose Microbiology Infectious Disease. 41: 139-142.
7- Dueger, E. L., Verastegui, M., Gilman, R. H. (2003): Evaluation of the enzyme linked immunoelectrotransfer blot (EITB) for ovine hydatidosis relative to age and cyst characteristics in naturally infected sheep. Veterinary Parasitology. 114: 285-293.
8- Ghorbanalinezhad, E., Assmar, M., Piazak, N., khabrii, A. (2001): Development of a new ELISA kit for the diagnosis of hydatidosis in human. Iranian Journal of Public Health. 30: 67-70.
9- Kittelber, R., Reichel, M. P., Jenner, J. (2002): Evaluation of three enzyme- linked immunosorbent assays (ELISA) for the detection of serum antibodies in sheep infected with Echinococcus granulosus. Veterinary Parasitology. 110: 57-76.
10- Lightowlers, M. W., Gottstein, B. (1995): Echinococcosis hydatidosis: antigens immunological and molecular diagnosis. In: Thompson, R.C.A. Lymbry, A.J. (eds) Echinococcosis and Hydatid Disease. Wallingford, UK: CAB International. 355 – 410.
11- Nasrieh, M. A., Abdele-Hafez, S. K. (2004): Echinococcus granulosus in Jordan assessment of various antigenic preparations for use in the serodiagnosis of surgically confirmed cases using enzyme immunoassay and the indirect heamagglutination test. Diagnose Microbiology Infectious Disease. 48: 117-123.
12- Parija, S. C. (1998): A review of some simple immunoassays in the serodiagnosis of cystic hydatid disease. Acta Tropica. 70: 17-24.
13- Rogan, M. T., Craig, P. S., Zeyhle, E. (1991): Evaluation a rapid of Dot-ELISA as a field test for the diagnosis of cystic hydatid disease. Transection of Royal Society Tropical Medicine and Hygiene. 85; 773-777.
14- Rokni, M. B., (2009): Echinococcosis/ hydatidosis in Iran. Iranian Journal Parasitology. 4: 1-17.
15- Romia, S. A., Youssef, M. E., Handoussa, A. E. (1992): Dot-ELISA as a diagnostic test in hydatid disease. Journal of Egypt Society Parasitology. 22: 603-610.
16- Sbihi, Y., Rmigui, A. (2000): Comparative sensitivity of six serological test and diagnostic value of ELISA using purified andtigen in hydatisosis. Journal Clinical Laboratory Annual. 15: 14-18.
17- Singh, B. P, Dhar, D. N. (1989): Indirect fluorescent antibody test for the detection of antibidies to Echinococcus granulosus in experimentally infected pups. Veterinary Parasitology. 28: 185-190.
18- Siavashi, M. R., tagerkhani, H., Rezaei, K. (2005): Comparision of Dot- ELISA and sandwich ELISA diagnosis tests in detection of human hydatidosis. Iranian Biomedical Journal. 9: 91-94.
19- Swarna, S.R., Parija, S.C. (2008): Dot-ELISA for evaluation of hydatid cyst wall, protoscoleces and hydatid cyst fluid antigens in the serodiagnosis of cystic echinicoccosis. Review Institute Medicine Tropical. 50: 233-236.
20- Verasteugi, M., Moro, P., Guevara, A., Rodrizvez, T., Miranda, E., Galman, R. J. (1992): Enzyme linked immunoelectrotransfer blot test for diagnosis of human hydatid disease. Journal of Clinical Microbiology. 30: 1557-1561.
_||_