Effect of Zeolite on microbial load of intestine in broilers
Subject Areas : Journal of Large Animal Clinical Science Research(JLACSR)
Keywords: zeolite, litter, broilers, Intestinal microbes,
Abstract :
In this research, effects of diet containing 0.5, 1 and 1.5 percent aminabad Qom zeolite and 1 percent commercial zeolite(anzemite) investigated on microbial load of intestine broilers. 400 day old male broiler chicks were bought and were allocated to 20 pens(5 treatments with 4 repetitions) equally. The control treatment got basal diet only. All chicks had kept in the same condition. In 42 day, 2 chicks to be butchered from each pen and sampling was done from the intestine of them. The samples were cultured in BA and EMB by serial dilution method .Colonies was accented after 24 h in 37˚. Measured PH of intestinal samples . E.coli were isolated from intestinal samples. They were tested antibiogram. The results showed that supplementation with zeolite don't make a change in ph compared with the control diet, and that chicks fed with zeolite didn't showed significant change in total microbial load . Supplementation with zeolite significantly reduced the counts of Entrobacteriaceae in the intestine(p<0.05). Antibiogram test showed that lowest resistance related to Gentamycin and highest resistance related to Penicillin and Erythromycin.
1- افشار مازندران، ن.، رجب، ا (١٣٨١): پروبیوتیکها و کاربرد آنها در تغذیه دام و طیور. چاپ دوم.انتشارات نوربخش، تهران ص90-35
2- فرخوی، م. (1375): راهنمای کامل پرورش طیور. چاپ سوم. واحد آموزش و پژوهش معاونت کشاورزی سازمان اقتصادی کوثر، تهران ص105-53
3- Akond, M., Hassan, S., (2009): Antibiotic resistance of Escherichia Coli isolated from poultry and poultry environment Bangladesh, A.J.Envi.Sci 5(1):47-52,
4- Doetkott, D.M., Nolan, L.K., Giddings, C.W., Berry, D.L., (1996): Large Plasmids of avian Escherichia coli isoltes. Avian Dis. 90: 927-930.
5- Gross, W.G., (1994): Diseases due to Escherichia coli in poultry. In Escherichia coli in domestic animals and humans. Edited by CL Gyles. CAB International,Wallingford, UK. PP: 237-259
6- Gunal,M., Yayli,G., Kaya,O., Sulak, O.,(2006):The effects of antibiotic growth promoter,probiotic or organic acid supplementation on performance, intestinal microflora and tissue of broilers.Int.Poult.Sci.,5:149-155
7- Harrigan, W.F., (1998): Laboratory methods in food microbiology, 3rd edn., academic press, San Diego, pp.432.
8- Kubena, L.F., Harvey, R.B., Phillips, T.D., Clement, B.A., (1993): effect of hydrated sodium calcium alummosilicates on aflatoxicosis in broiler chickens. Poult. Sci. 72:651.
9- Mumpton, F.A., (1994): mineralogy and geology of natural zeolite .department of the earth science. New York, usa.
10- Olver, M.D.,(1997): Effect of feeding clinoptilolite( zeolite) on the performance of three strains of laying hens. Br. Polt.Sci.38:221.
11- Santos, A.A, Ferket,P., (2006):Effect of nutrition on gut microflora colonization. 33rd Annual Carolina Poultry Nutrition Conference, September, 26-27
12- Sharada, R., Wilfred Ruban, S., (2010) : isolation,characterization and Antibiotic resistance pattern of Escherichia Coli isolated from poultry, A.E. J.Sci. R. 5(1):18-22.
13- Tomlinson, A.A.G., (1998): modern zeolites, steructure and function in detergents and petrochemicals. Trans tech ltd. Uk. 1-16.
14- Vest , L. Shutze, J., (1984): Influence of feeding zeolites to poultry under field condition. . In zeo . Agriculture: use of natural zeolites in Agriculture and aquaculture. W.G.Pond and F.A. Mumpton. Eds., Westview press, Boulder, Colorado.211.
15- www. Featured Poultry Articles At Poulvet.com
16- Xia, M., Hu, S., Xu, C.H., (2004):Effects of copper-bearing montmorillonite growth performance digestive, enzyme activities, and intestinal microflora and morphology of male broilers. Poult. Sci. 83;1868-1875.
_||_