Determination of serum haptoglobin reference value in apparently healthy dromedary camels (Camelus dromedaries) in the central regions of Iran

Subject Areas : Journal of Large Animal Clinical Science Research(JLACSR)

Received: 2021-08-15 Accepted : 2021-08-15 Published : 2014-04-21

Keywords: haptoglobin, Acute phase protein, Reference Value, Camelus dromedaries,

Abstract :

Acute phase proteins (APP) are a class of serum protein concentration in response to inflammation, trauma, and neoplasia increases significantly. Haptoglobin (HP) is one major positive acute phase proteins, that have low concentration in the serum of healthy animals, but with its concentration increase over 100- fold on stimulation. Concentration remained high for about 24-48 hours and during the recovery phase begins to decrease rapidly. In order to determine the reference range for serum haptoglobin concentration camels Iranian dromedary camels jugular vein sixty Iranian Central aged one to five years in both sexes were taken in the spring and summer of 1389. Blood samles were collected from jugular vein and haptoglobin value was measured by using colorimetric method with Ireland commercial kits. Age and sex had no significant effect on the haptoglobin value of clinically healthy Iranian dromedary camels (p>0.05). There was no significant correlation between age and serum haptoglobin concentrating in Iranian doromrdary camels (p>0.05). Reference value of serum haptoglobin in clinically healthy Iranian dromedary camels (Camelus dromedarius) was 0.54-1.04 mg∕ml (mean ±SD =0.79± 0.128).

References:

• Eaton, J. w., Brandt, P., Mahony, J. R., (1982). Haptoglobin: a natural bacteriostat. Science. (215): 691-693.


• Eckersall, P.D., (2008). Proteins, proteomics and dysproteinemias. In: Kaneko, J.J, Harvey, J.W, Bruss, M.L., (eds) Clinical biochemistry of domestic animals, 6th ed. Academic Press, San Diego: 117-55.


• Eckersall, P.D., Bell, R., (2010). Acute phase proteins: Biomarkers of infection and inflammation in veterinary medicine. Veterinary Journal. (185): 23–27.


• Feldman, B.F., Zinkl, J.G., Jain, N.C., (2000). Schalm's veterinary hematology. Lippincott Williams, Wilkins, philadelphia, 891–896.


• Kushner, I., Mackiewicz, A., (1987).  Acute phase proteins as disease markers. Disease Marker. (5): 1-11.


• Hirvonen, J., Eklund, K., Teppo, A.M., Huszenicza, G., Kulcsar, M., Saloniemi, H., Pyorala, S., (2000). Acute phase response in dairy cows with experimentally induced Escherichia coli mastitis, Acta Veterinaria Scandinavica, (40): 35-46.


• Hiss, S., Mielenz, M., Bruckmaier, R. M., Sauerwin, H., (2004). Haptoglobin concentrations in blood and milk after endotoxin challenge and quantification of mammary Hp mRNA expression. Journal of Dairy Science. (87): 3778-3784.


• Lipperheide, C., Goth, C., Petersen, B., Sommer, H., (1997). Nephelometric assay of haptoglobin in blood plasma from cattle, pigs and horses. Tierarztl. Umsch. (52): 420-426.


• Mark, V., Crisman, W., kentScarratt, K., Zimerman, L., (2008). Boold proteins and Inflammation: Veterinary clinical Equine. (24): 285-297.


• Merdev, B., (1981). Some problems of camel breeding in turkemnia kaneuod stvoikonny: Sport animal. Animal Breeding. 12(7): 22-24.


• Minka, N.S., Ayo, J.O., (2007). Effects of loading behaviour and road transport stress on traumatic injuries in cattle transported by road during the hot-dry season. Livest Science. (107):91–95.


• Murata, H., Shimada, N., Yoshioka, M., (2004). Current research on acute Phase Proteins in veterinary diagnosis, An Overview the Veterinary Journal. (168): 28-40.


• Nakagawa, H., O. Yamamoto, S. Oikawa, H. Higushi, A. Watanabe, N., (1997). Detection of serum haptoglobin by enzyme-linked immunosorbent assay in cows with fatty liver. Research in Veterinary Science, (62): 137-141.


• Nazifi, s., Rezakhani, A., Koohimoghadam, M., Esmailzadeh, Z., (2008). Evaluation of serum haptoglobin in clinically healthy cattle and cattle with inflammatory diseases in Shiraz, a tropical area in southern Iran. Bulgarian Journal of Veterinary Medicine, 11(2): 95-101.


• Niewold, T.A., Toussaint, M.J.M., Gruys, E., (2003). Monitoring health by acute phase proteins, Proceedings of the Fourth European Colloquium on Acute Phase Proteins, 57-67, Segovia, Spain.


• Nowroozi-Asl, A., Nazifi, S., Bahari, A., (2008). Determination of serum haptoglobin reference value in clinically healthy Iranian fat-tailed sheep. Iranian Journal of Veterinary Research, Shiraz University, 9(2):171-173.


• Paltrinieri, S., (2008). The feline acute phase reaction. Veterinary Journal. (177): 26–35.


• Petersen, H.H., Nidsen, J.P., Heegaard, P.M.H., (2004). Application of acute phase protein measurements in veterinary clinical chemistry.Veterinary Research. (24):163-87.


• Safi, S., Khoshvaghti, A., Jafarzadeh, S.R., Bolourchi, M., Nowrouzian, I., (2009). Acute phase proteins in the diagnosis of subclinical mastitis. Veterinary Clinical Pathology. (38):471-476.


• Salonen, M., Hirvonen, J., Pyorala, S., Sankari, S., Sandholm, M., (1996). Quantitative determination of bovine serum haptoglobin in experimentally induced Escherichia coli mastitis. Research Veterinary Science. (60): 88-91.


• Taira, I., Fujinaga, T., Okumura, M., (1992). Equine haptoglobin isolation, characterization and the effects of ageing, delivery, inflammation on the serum concentration. Journal of Veterinary Medical Sciences, (54): 435-442.


• Vojdanifar, N., Safi, S., Rahimiforoushani, A., Khaledi, S., (2012). Study of changes in serum amyloid A (SAA) and haptoglobin during inflammation induced by turpentine injection in Caspian miniature horses. ch - Journal of Veterinary Medicine. Islamic Azad University, SanandajBran6(1):1-1


• Whicher, J.T., Westacott, C.I., (1992). The acute phase response, In: Biochemistry of inflammation, Kluwer, Academic, London, United Kingdom. 243-271.