Seroprevalence of Toxoplasmosis in Cattle, Sheep and Goat, Using ELISA and Indirect Immunoflorescent antibody (IFA) Methods in Lorestan Province

Subject Areas : Journal of Large Animal Clinical Science Research(JLACSR)

Received: 2021-08-22 Accepted : 2021-08-22 Published : 2014-08-23

Keywords: Lorestan, ruminants, Elisa, Toxoplasmosis, Indirect Immunoflourecence antibody,

Abstract :

Toxoplasmosisa zoonotic disease caused by the intracellular protozoan of Toxoplasmagondii, has not been examined in Lorestan yet; so this study aimed at the prevalence rate of IgG in the domestic ruminants, based on the variable factors of species, sex and geographic region. Therefore, from October to February 2012, 760 blood samples (174 samples from Cattles, 398 samples from sheep, and 188 samples from goats) were collected from the slaughterhouses of Khoramabad, Boroujerd and Aligoudarz. The serum samples of the sheep and goats were examined by indirect ELISA method, and the results were calculated based on the ratio of the the absorbance value of the samples to the positive control, so that ≤30% was considered as negative, and ≥50% as positive. The Cattle serum samples were examined via Indirect Immunoflourecence antibody test, and the titer≥1:16 were considered as positive. In this study, the IgG prevalence rate in the cattle, sheep and goat was respectably 28.73%, 53.01%, and 51.66%; and the statistic analysis of the results, demonstrated that based on the sex and species, there was a considerable statistic difference, so the Lowest infection ratewasseen in the cattle, and the most infection rate in the sheep, so the prevalence rate in females was higher than males but no statistic difference was seen geographically (p<0.05). Since, there is a high frequency rate of Toxoplasmosis in the sheep and goats of Lorestan, like other parts of the country, and the importance of their meat which is considered as amain source of protein, public informing and training is essential topreventethe disease.

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