Comparing the human IgG class and subclasses and IgE responses against antigen B, cattle, sheep and mice crude hydatid cyst Fluid antigens By ELISA method
Subject Areas : Journal of Large Animal Clinical Science Research(JLACSR)
Keywords: Sheep, cattle, Mice, Elisa, Hyadatid cyst, Crud antigens,
Abstract :
Finding a human based antigen is either time consuming, hazardous, expensive or very difficult and if applicable it is in very low scale. Therefore the aim of the current study was to compare the human responses against antigen B, cow, sheep and mice crude hydatid cyst fluid (HCF) antigens of Echinococcus granulosus. Hydatid cysts obtained from lungs and livers of sheep and cattle slauthered from local abattoirs in Ilam city, and was used as the source of antigen either for the SDS, Western blotting or ELISA analysis or for experimentally infecting the mice. 30 samples of sheep and 30 samples of cattle hydatid cyst fluid antigen were used as the source of antigen and also to experrimentally infect the mice. human sera samples were collectesd from surgery section of hospitals in Ilam city . IgG and IgE responses were measured using ELISA in case-control study design. Each response was comparly to the others. Results were analysed using Anova and Post Hoc methods. HCF recovered from cysts was centrifuged at 10000 rpm for 5 minutes. The supernatant was dialysed against PBS, freezed-thawed and used as crude HCF.There was an increasing in IgG4 subclass level against cattle hydatid cyst crude antigen with 10.2 times against cut off and B antigen with 9 times against cut off. In this analysis all IgG class and subclasses and IgE antibody responses showed a higher mean OD than that of the cut off, indicating that all human sera from patients with hydatidosis reacted againstcow, sheep and mice crude hydatid cyst fluid antigens and also antigen B. There was an acceptable cross reaction of human immune responses against cattle, sheep, mice and B with that of human using ELISA. Mice antigen is suitable for designing a diagnostic kit. Human total IgG and IgG4 had the highest responses against these antigens comparing to other antibodies.Hyadatid cyst fluid antigen of both cattle and sheep were significantly useful in serodiagnosis of hydatidosis similar to that of human and therefore can be used in hydatid cyst diagnosis kit using ELISA.
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