The Impact of Copper on Histhopathological Point of Gonad, Liver and Muscle Tissues of Cyprinus Carpio

Subject Areas : Journal of Large Animal Clinical Science Research(JLACSR)

Received: 2021-07-10 Accepted : 2021-07-10 Published : 2009-06-22

Keywords: Copper, liver, Muscle, Cyprinus carpio, Gonad, L50,

Abstract :

In this research, the impact of copper on Cyprinus carpio was assessed in laboratory conditions. At first, L50-96h amount of copper was calculated on finger-length and mature fish, resulting in determination of 0.27 and 2.3 mg/lit. Then, probable microscopic damages of gonad, liver and muscle tissues of Cyprinus carpio, exposed to copper with concentration ranges of 2_6 mg/lit, were assessed. The cross sections were studied microscopically from histhopathological point of view.  The achieved results showed that when the liver was exposed to copper, fat degeneration, bleeding and hemorrhage would happen, as well as necrosis, invasion of lymphocytes and liver capsule inflation at higher concentration. Degeneration of the ovary and penetration of inflated cells in testis as well as homocydrin residue inside the testis and the increase of coagulated tissues were observed. No damage was observed in the muscles. In addition, the amount of copper bioaccumulation in liver, gonad and muscle tissues was determined with atomic absorption method at three sub lethal concentrations of copper (0.1, 0.5, 1 mg/lit). Analytical results show minimum concentration of copper accumulation in muscles and maximum concentration of copper in the liver of Cyprinus carpio.

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