Bacteriology and antibiogram of clinical and subclinical mastitis in dairy herds of Sanandaj city
Subject Areas : Journal of Large Animal Clinical Science Research(JLACSR)
Keywords: Sanandaj, Antibiogram, Bacterial mastitis,
Abstract :
Mastitis causes great economic losses including reduction of milk production and quality as well as milk microbial contamination is considered as a means of disease transmission to humans. Therefore investigating various aspects of the disease can be effective in controling and decreasing damages due to the disease. In this study, 200 samples of raw milk from cows with clinical mastitis or cows which showed different degree of positivity in the California mastitis test were tested. After colony identification, antibiotic susceptibility test was performed by disk diffusion method. Cultures were positive in 152 cases (%76) and negative in 48 cases (%24). Highest frequency belonged to species Micrococcus luteus (%39.5), Micrococcus varians (%14.5), Staphylococcus epidermidis (%10.5), E.Coli (%6.6), Staphylococcus aureus (%2.6) and lowest frequency belonged to the Serratia (%1.3). In the antibiogram test the most sensitivity of agents genus was to contamination was Gentamycin (%80.31), Enrofloxacin (%77), Ciprofloxacin (%66) and Oxytetracyclin (%48) and the least sensitive was to Cefixime (%9.9). Results derived from the antibiogram tests in the study indicates the necessity of similar periodic studies which can be considered a regional therapeutic programme.
1- هاشمی، م. و پایدار، ع. (1387): بررسی حساسیت و مقاومت آنتیبیوتیکی باکتریهای جدا شده از نمونههای شیر خام مشکوک به ورم پستان در گاوداریهای استان فارس. پانزدهمین گنگره دامپزشکی ایران.تهران.
2- یاراحمدی، ب. و مهدوی، ح. و مؤیدینژاد، ا. (1387): بررسی آلودگی بار میکروبی کل، کلیفرم و اشریشیا کلی شیر خام از مرحله دوشش تا تحویل به کارخانه در استان لرستان. فصلنامه علمی-پژوهشی دانشگاه علوم پزشکی لرستان، دوره دهم، شماره 3.
3- Awale, M.M., Dudhatra, G.B., Avinash, K., Chauhan, B.N., Kamani, D.R., Modi, C.M., (2010) : Bovine Mastitis: A Threat to Economy. 4:36-39.
4- Bramley, A.J., (1982): Sources of Streptococcus uberis in the dairy herd isolation from bovine faces and from straw bedding of cattle. Journal Dairy Research.49: 369-373.
5- Bramley, A.J., McKinnon, C.H., (1990) : The microbiology of raw milk. In Dairy Microbiology. The Microbiology of Milk. 2ed. R. K. Robinson, ed. Elsevier Science Publishers, London, United Kingdom 1: 163-208.
6- Buragohain, J., Dutta, G.N., (1998) : Inactivation of chloramphenicol by Staphylococcus aureus biotype C from humans & animals. Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases.19: 53-59.
7- Ehsani, A., (1999) : Educational workshop of raw milk improvement methods in country. Animal affairs construction jihad. Department of animal and poultry changing industries 59-62.
8- Elango, K.A., Doraisamy, G., Rajarajan, G., Kumaresan, G., (2010) : Bacteriology of sub clinical mastitis and antibiogram of isolates recovered from cross bred cows. Indian Jurnal Animal Research. 44 (4) : 280 – 284.
9- Haque,1., Sahay1, S., Singh, K., Roy, B K., (2013) : Bacteriology and antibiogram of Bovine mastitis in Ranchi and Visiniti. International Journal of Agriculturl Science & Veterinary Medicine.1 (1).
10- Hawari Azmi, D. Fawzi, A., Anim, J., (2008) : Prevalence and Distribution of Mastitis Pathogens and their Resistance against Antimicrobial Agents in Dairy Cows in Jordan. American Journal of Animal and Veterinary Sciences. 3: 36-39.
11- Mahantesh, M., Kurjogi, A. Basappa B., Kaliwal, G., (2011) : Prevalence and Antimicrobial Susceptibility of Bacteria Isolated From Bovine Mastitis. Advances in Applied Science Research. 2 (6) :229-235
12- Martin, R.P.Lindert, O. Hausmann, D. Daniel, WG., (2006) : Improvement in the diagnosis of abscesses associated with endocarditis by transesophageal echocardiography. Hygiene Alimentar. 20: 139-143.
13- Paul, W.M., (2000): Indian Journal Of Animal Health. 39 (2): 79- 81.
14- Poelarends, J., Hogeveen, H., Sampimon, O.C., Sol, J., (2001) : Monitoring subclinical mastitis in Dutch dairy herds. Proceedings of the second international symposium on mastitis and milk quality, Vancouver, British Columbia. 145-149.
15- Quinn, P.J., Carter, M.E., Makey, B.K., Carter, G.R., (1994): Clinical Veterinary Microbiolgy Published by Wolfe Publishing.PP, 331.
16- Radostis,O.M., Gay, C., Hinchclff,K. W., Constable, P.D., (2007) : Veterinary Medicine.(10th Ed.) Saunders, Philadelphia.PP. 5: 310-315
17- Shrirame, K.R., Bhardwaj, S., Kalorey, D.R., Harne, S.D., (2002) : Study of pathogenicity markers of Staphylococci from subclinical mastitis in Proceedings of Round table Conference. Feb 4-5, Nagpur India.pp. 18-22.
18- Sudhakar, P., Awandkar, V., Khode, M., Mangesh, S., Mendhe, S., (2009) : Prevalence and Current Antibiogram Trend of Mastitic Agents in Udgir and its Visinity, Maharashtra State, India. International Journal of Dairy Science, 1-5.
19- Tenhagen, B.A., Koster, G., Wallman, J., Heuwieser, W., (2006) : Prevalence of mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Brandenburg. J Dairy Sci 89: 2542-2551.
_||_