Seroprevalence of avian Metapneumovirus and Influenza (H9N2 subtype) infection in broiler chickens with respiratory syndrome in Isfahan Province

Subject Areas : Journal of Large Animal Clinical Science Research(JLACSR)

Received: 2021-08-01 Accepted : 2021-08-01 Published : 2011-09-23

Keywords: Broiler, Influenza, Metapneumovirus, Turkey rhinotracheitis virus,

Abstract :

The infection of avian pneumovirus (Avian Metapneumovirus) is characterized with respiratory signs and rhinotracheitis in turkey (TRT) and swollen head syndrome (SHS) in chickens and may cause drop in egg production in layer and breeders. Therefore, in present study the infectivity of avian pneumovirus was evaluated and the co-infection of avian pneumovirus with avian influenza was investigated in broiler chickens flocks with respiratory signs. In this study, 360 serum samples were collected from 30 broiler chicken flocks, with respiratory signs and daily mortality rate over 1%, from different areas in Isfahan province. All flocks divided into 2 groups: vaccinated and nonvaccinated against avian influenza (AI). Serum samples were tested with avian pneumovirus enzyme-linked immunosorbent assay (ELISA) kit. Also, AI haemagglutination inhibition (HI) titre was determined in all aforementioned flocks. The results showed that out of 360 broiler chickens, 320 (88.9%) were positive for avian pneumovirus antibody. The seroprevalence of pneumovirus in vaccinated flocks against AI was lower than nonvaccinated flocks and 82.5% of broiler chickens in nonvaccinated flocks were positive for AP and AI antibodies. Therefore, it seems that controlling usual respiratory diseases (eg. AI) may result in controlling avian pneumovirus. Regarding high prevalence of avian pneumovirus in broiler chickens flocks with respiratory signs, it is important to investigate pathogenesis of avian pneumovirus to design a suitable strategy for controlling this infection.

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