Serological survey of Bluetongue disease in sheep of Sanandaj city by ELISA
Subject Areas : Journal of Large Animal Clinical Science Research(JLACSR)
Keywords: Sheep, Elisa, Bluetongue,
Abstract :
Bluetongue is a transferable non-contagious disease that mostly affects sheep. Stomatitis, Rhinitis, Enteritis, Lameness and Abortion are the characteristics of this diesease. In this research 300 blood serums were chosen in order to examin serologically bluetongue disease in the sheep of Sanandaj city.The villages were chosen through random systematic method from sanandaj among four theoretical zones of northeast, north west, southeast and southwest in 1386. The samples were chosen through simple random method and examined by A competitive enzyme- linked immunosorbent assay (cELISA) method. The results showed that 19.3% of the sheep had positive titre. The percent of positive titre rate of bluetongue virus in the seasons of winter, autumn, spring and summer were 29.3, 20, 16, and 12%, respectively. The amount of positive titre was mostly related to 3 to 5 ages range and the number of positive titer observed in north areas of sanandaj was many more than south areas. Season and the area of sampling are meaning in appearing the positive titre disease in sanandaj. Gender and mutual effect and season, age and the area are meaning ful too. These results showed that most probably the virus is strolling in north areas. Besides, it is necessary to do something to reduce infection to the bluetongue virus in north areas of sanandaj, and cELISA shoud routinely used for the detection of antibodies against BTV in these local breeds of sheep.
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