Molecular study of hydatid cyst strains isolated from domestic ruminants in Isfahan province, Iran
Subject Areas :
سید رضا حسینی
1
*
,
میلاد حمزه علی طهرانی
2
1 - گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
2 - گروه پاتوبیولوژی ، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران، ایران
Keywords: Echinococcus granulosus, hydatid cyst, strain determination, Isfahan, Iran,
Abstract :
Echinococcosis is one of the common parasitic diseases between humans and animals, whose symptoms appear with the long-term growth of the babies of this cestode in the body of the intermediate host, generally ruminants, and humans are known as the accidental and final host for this parasite. Although this disease in It is not reported in some countries, it has a global distribution, and considering the zoonotic importance of this disease, it is necessary to know the strains and determine the level of infection with this parasite in the intermediate hosts, because the interruption of the developmental cycle of the parasite in the intermediate hosts and the final host is very important and also this parasite has a number of species or strains within the species that this genetic diversity can be potentially important in epidemiology and disease control and making vaccines and therefore the study of mitochondrial DNA sequence and phylogenetic studies based on The DNA of this cestode is very important. 244 samples from ruminants (sheep, cows, camels, and goats) slaughtered in the industrial slaughterhouses of Isfahan province (61 infected liver samples, including two groups of 31 males and 30 females) were collected during the period from April to September of 2022 and After preparing protoscoleces from the existing hydatid cysts, DNA was extracted using a DNA extraction kit, PCR reaction using primers BD1 (Forward) and 4S (Reverse) which can amplify the ribosomal genome of the rDNA-ITS1 region. and led to the production of a PCR product with a length of 1000bp was used and subjected to enzymatic digestion (PCR-RFLP) using endonuclease enzymes AluI, RsaI, HpaII, and TaqI and finally the results were obtained using SPSS version 24 software and with Chi-square test (X2) was used for statistical analysis and the significance level of the research (P<0.05) was considered. The results show definite contamination with E. granulosus and sheep strain (G1) caused by the sheep-dog cycle in all the samples obtained from the liver of ruminants, and in the comparison and statistical analysis, there is a significant difference between the type of contamination with different strains between There was no male or female sex. This study can confirm the previous results about the predominance of the sheep strain in Iran, so it is recommended to pay special attention to this issue in order to control and prevent the transmission of this parasite in the sheep-dog cycle.
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