The effects of Saponin derived from Tribulus terrestris on the activity and structure of α-Glucosidase
Subject Areas : biophsics
sama alijani
1
,
Azadeh Hekmat
2
,
sara Khavarynejad
3
1 - Faculty of biology Department of biopHysics
2 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Ira
3 - Department of Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Karaj, Iran
Keywords: Spectroscopy, Saponin, Inhibitor, α-glucosidase, Tribulus terrestris,
Abstract :
α-Glucosidase is a kind of hydrolase enzyme that catalyzes the hydrolysis of non-reducing terminal carbohydrates into α-glucose. Inhibition of this enzyme prevent the glucose absorption. Compounds that can inhibit α-glucosidase are widely used for the treatment of type 2 diabetes. Tribulus terrestris is a medicinal plant which is distributed around the world. Saponins in this plant are responsible for its biological activities. In the research, the α-glucosidase inhibitory activity of saponins extracted from Tribulus terrestris has been studied by ELISA reader, UV-Visible, and circular dichroism (CD) spectroscopies. The results of ELISA reader displayed that Saponins have functioned as uncompetitive inhibitory effects on α-glucosidase. The half maximal inhibitory concentration (IC50) value were 38 µM for saponin. The absorption of α-glucosidase increased with the addition of saponin concentrations. Comparison of the α-glucosidase and α-glucosidase-saponin complex displayed that the content of the α-helix structure decreased and the content of the β-sheets and random coils structure increased. Collectively, the results showed that saponins extracted from Tribulus terrestris can disrupt the α glucosidase enzyme structure and inhibit the enzyme activity. Altogether, the saponins extracted from Tribulus terrestris can be a good candidate for Type II diabetes treatment.
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