Extraction and measurement of β-Carbolines and evaluation of antioxidant properties of leaf and seed extract of Spand (P.harmala) of Zahedan
Subject Areas : phytochemistry
Saeedeh Fahimi
1
(Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran)
Shahrbanoo Oryan
2
(1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2. Department of Animal Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran)
Ramesh Ahmadi
3
(Department of Animal Sciences, Faculty of Basic Sciences, Islamic Azad University of Qom, Qom, Iran)
Akram Eidi
4
(Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran)
Keywords: β-carboline, High Performance Liquid Chromatography (HPLC), Spand (p.harmala), phenolic compounds,
Abstract :
β-Carbolines are an alkaloid containing indole group that easily passes through the blood-brain barrier and change the concentration of neurotransmitters, as well as a range of neurophysiological and toxic effects, including effects on body temperature, seizures, anti-depressant activity, vascular dilatation, anti-cumulative effects of platelets and effects on appetite. The aim of this study was to extract and measure β-Carboline and also phenolic compounds of Spand (p.harmala) collected from Zahedan. Further phytochemical analysis of extracts was carried out and the quantitative and qualitative analysis included identification of anthocyanins, flavonoids, tannins, anthraquinones, an antioxidant test of extract, FT-IR analysis of extract, quantitative and qualitative analysis of HPLC extract and alkaloids. The results showed that although the antioxidant properties of methanolic extract from leaves are higher (due to the presence of flavonoids and phenolic compounds) than the methanolic extract seeds, the amount of β-Carboline in the seeds of this plant (p.harmala) is higher.
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