Carbonic anhydrase activity investigation in the present of Sodium oxalate and Ethylendiamine
Subject Areas : biophsics
Yoones moosavi
1
,
Azadeh Hekmat
2
,
Mahdi Alijanianzadeh
3
1 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of bio science and biotechnology, Malek Ashtar university of technology, Tehran, Iran
Keywords: Lineweaver-burk plot, Sodium oxalate, Carbonic anhydrase, Ethylene diamine,
Abstract :
Introduction: Carbonic anhydrases are ubiquitous zinc containing metalloenzymes which catalyze the interconversion of bicarbonate and CO2. This enzyme is involved in various vital physiological processes related to respiration, renal acidification, pH homeostasis, electrolyte secretion, calcification, neurotransmitter and some biosynthetic reactions. In this study the inhibitory effects of sodium oxalate and ethylene diamine on carbonic anhydrase activity were studies. Methods: The enzyme activity were measured in the presence and absent of ethylene diamine and sodium oxalate in various concentrations. The initial rates were measured in each assay. Then the kinetics parameters and IC50 were measured. Results: The results of double reciprocal lineweaver-burk plots showed the combination inhibitory effect on carbonic anhydrase activity for ethylene diamine and uncompetitive inhibitory effect on carbonic anhydrase activity for sodium oxalate. Furthermore, the Km, Vmax, IC50 and Ki displayed that sodium oxalate is stronger inhibitor than ethylene diamine. Conclusion: Altogether, due to essential role of carbonic anhydrase in many physiological processes, it is important to consume sodium oxalate and ethylene diamine very cautiously.
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