Kinetic modeling the behavior of the enzyme glucose oxidase immobilized on a carrier tragacanth

Subject Areas : biochemical

golnar sari ¹ (student)
مریم اوتادی ² (استادیار، گروه مهندسی شیمی، دانشکده فنی و مهندسی، دانشگاه آزاد اسلامی، واحد تهران مرکزی، تهران، ایران)

Received: 2016-10-26 Accepted : 2017-01-04 Published : 2017-05-22

Keywords: :Enzymes and enzymatic activity, modeling enzyme kinetics of the enzyme, glucose oxidase immobilized enzyme,

Abstract :

Abstract The glucose-oxidase enzyme is widely used in industry. Glucose oxidase is an oxido-reductase enzyme that catalyses the oxidation of glucose to hydrogen peroxide and gluconic acid. In this study, the glucose oxidase enzyme, its structure and function, tragacanth polymer, different methods of enzyme immobilization and statistical modeling methods have been introduced. To investigate the enzyme activity of glucose oxidase using design of experiments (DOE) and response surface methodology (RSM), the results of 19 experiments, have been conducted in previous research, were used. For modeling and optimization of enzymatic activity, three variables of pH, temperature and immobilization time were selected and examined using the Design Expert software. Then, based on statistical modeling, a correlation was proposed that be able to determine the enzyme activity of the glucose oxidase. Using response surface methodology, maximum enzyme activity (1116.2 μmol/min) of the glucose oxidase was achieved at pH of 5.98, 37 ° C and 33.28 minutes. Finally we observed that our model results are accurately agreed with the experimental results.