Evaluation of the effect of priming on germination and growth characteristics of sugar beet cultivars (Beta Vulgaris L) under salinity conditions
Subject Areas :Seyed GholamReza Salehi 1 , حشمت امیدی 2 , Mehdi Hasani 3 , Mohammad Hosein Bijeh Keshavarzi 4
1 - MSc, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahed University, Tehran, Iran
2 - استادیار، گروه زراعت و اصلاح نباتات، دانشکده علوم کشاورزی، دانشگاه شاهد، تهران.
3 - Assistant Professor, Ph.D., Sugar Beet Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
4 - PhD student, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahed University, Tehran, Iran
Keywords: Hydropriming, Potassium nitrate, Sugar beet cultivars, Germination speed,
Abstract :
This study aims to investigate the effect of priming treatments (control without priming, hydropriming, priming with 0.25% potassium nitrate and priming with 0.5% potassium nitrate) on the germination characteristics of sugar beet cultivars and growth characteristics under five levels of salinity (0, 4, 8, 12, and 16 dS/m) on germination and seedling growth in four replicates in a petri dish in laboratory conditions as a factorial experiment based on a completely randomized design. The results showed that the effect of seed priming and salinity stress on the average germination time, germination speed coefficient, germination variance, germination uniformity, root length, stem length, stem and root dry weight, water content relatively, chlorophyll a, chlorophyll b and carotenoids were significant. The results showed that in all three genotypes, the number of germinated seeds decreased with the increase in salinity level, and in the control levels and the use of water as a priming factor, this decrease was moderated. With the increase of salt concentration up to 12 ds level, the relative water content increased sigmoidally and showed a relatively stable trend at two levels of 12 and 16 ds. In most of the investigated traits, Shokofa variety has shown less reaction than other genotypes. In the investigation of the reaction process of genotypes to the speed, variance and homogeneity of germination in prime and salinity levels, it has shown a decrease with increasing salinity concentration.
Abdollahian Noghabi, M., Sheykholeslami, R. and Babaei, B. 2005. Terms and meanings of technological quantity and quality of Sugar beet. Sugar beet Journal. 21: 101-104. (In Persian, abstract in English)
Akram-Ghaderi, F., Soltani, E., Soltani, A. and Miri, A.A. 2008. Effect of priming on response of germination to temperature in cotton. Journal of Agricultural Sciences and Natural Resources. 15: 44-51. (In Persian).
Alavi, Z.S., Roushanfekr, H., Hasibi, P. and Mesgarbashi, M. 2012. Effect of Osmo and Hydro-priming on the rate and percent of germination of sugar beet genotypes under salt stress. Proc. Second Conf. Seed Sci. Technol. Mashhad.
Bahmani, M., Jalali, Gh. and Tabari, M. 2014. Effects of halopriming on germination traits of medicinal plant caper small shrub (Capparis spinosa var. parviflora) seeds. Arid Biome Scientific and Research Journal. 4(1): 79-82. [In Persian with English summary].
Balochi, H.R. and Ahmadpour Dehkordi, S. 2013. Effect of different seed priming on germination traits in black cumin (Nigella sativa) under salinity stress. Journal of Plant Production. 20(3): 1-25. [In Persian with English summary].
Beheshti, A., Tavakoli, H.R. and Kochaki, A. 2000. Combination effect of salinity stress and temperature on the germination of Alfalfa cultivars. Agricultural Sciences and Technology Journal. 14(1): 71-79.
Bybordi, A. and Tabatabaei, J. 2009. Effect of salinity stress on germination and seedling properties in canola cultivars (Brassica napus L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 37(2): 71- 76.
Cavusoglu, K. and Kabar, K. 2010. Effects of hydrogen peroxide on the germination and early seedling growth of barley under NaCl and high temperature stresses. EurAsian Journal of Bioscience. 4: 70-79.
Cha-um, S. and Kirdmanee, C. 2009. Effect of salt stress on prolin accumulation, photosynthetic ability and growth characters in two maize cultivars. Pakistan Journal of Botany. 41: 87-98.
Chen, K., Fessehaie, A. and Arora, R. 2011. Dehydrin metabolism is altered during seed osmopriming and subsequent germination under chilling and desiccation in Spinaciaoleracea L. cv. Bloomsdale: Possible role in stress tolerance. Plant Science. 48: 1-11.
Demir, I. and Mavi, K. 2004. The effect of priming on seedling emergence of differentially matured watermelon (Citrullus lanatus (Thunb.) Matsum and Nakai) seeds. Scientia Horticulturae 102: 467-473.
EL-Tayeb, M.A. 2005. Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regulation. 3: 215-225.
Farhoudi, R. and Khyamim, S. 2020. Evaluation of Iranian sugar beet commercial varieties under salinity stress in germination and establishment growth stages. Plant Process and Function. 9(36): 397-412
Farhoudi, R. and Sharifzadeh, F. 2006. The effects of NaCl priming on salt tolerance in canola (Brassica napus L.) seedlings grown under saline conditions. Indian Journal Crop Science. 1(1–2): 74-78.
Francis, G., Jhon, L., Jifon, S., Micaela, C. and James, P.S. 2002. Gas exchange, Chlorophyll and nutrient contents in relation to Na and Cl accumulation in sunburst mandarin grafted on different root stocks. Plant Science. 35: 314-320.
Fujikura, Y., Kraak, H.L., Basra, A.S. and Karssen, C.M. 1993. Hydropriming, a simple and inexpensive priming method. Seed Sci. Technol. 21: 639– 642.
Ghassemi-Golezani, K., Chadordooz-Jeddi, A., Nasrullahzadeh, S. and Moghaddam, M. 2010. Influence of hydro-priming duration on field performance of pinto bean (Phaseolus vulgaris L.) cultivars. African Journal of Agricultural Research. 5(9): 893-897.
Hossini, A. and Koochaki, A. 2007. The effect of different priming treatments on germination rate and percentage of four varieties of sugar beet seed. Journal of Iranian Agriculture Research. 9: 69-76 (In Persian).
ISTA. 2003. International Seed Testing Association. ISTA Handbook on Seedling Evaluation, 3rd ed.
Jamil, M. and ShekRha, E.S. 2008. Gibberellic acid (GA3) enhance seed water uptake, germination and early seedling growth in sugar beet under salt stress. Pakistan Journal of Biological Sciences. 10(4): 654-658
Kamali, M., Kharazi, M., selahverzi, Y. and Tehranifar, A. 2012. Effect of salycilic acid on growth and some morphophysiologic characteristics of Gomphrena globosa L. under salt stress. Journal of Horticultural Science. 26(1): 104-112. [In Persian with English summary].
Kaya, M.D., Okcu, G., Atak, M., Cıkılı, Y. and Kolsarıcı, O. 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annus L.). Europ. J. Agronomy. 24: 291-295.
Kaya, M.D., Okcu, G., Atak, M., Cıkılı, Y. and Kolsarıcı, O. 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annus L.). European Journal. Agronomy. 24: 291-295.
Khajeh-Hosseini, M., Powell, A.A and Bingham, I.J. 2003. The interaction between salinity stress and seed vigor during germination of soybean seeds. Seed Science and technology. 31(3): 715-725
Khayamim, S., Jahadakbar, M.R., Noshad, H., Rozbeh, F. and Zavieh Mavadat, L. 2014. Effect of salt stress on photosynthetic components of sugar beet in the greenhouse and field conditions. Journal of Sugar Beet. 30(1): 33-41.
Mori, S., Fujimoto, H., Watanabe, S., Ishioka, G., Okabe, A., Kamei, M. and Yamauchi, M. 2012. Physiological performance of iron-coated primed rice seeds under submerged conditions and the stimulation of coleoptile elongation in primed rice seeds under anoxia. Soil Science and Plant Nutrition. 58: 469-478.
Mousavi, S.A. and Omidi, H. 2017. Effect of Biomedical Treatments on Germination, Growth and Physiologic Parameters of Paper Pumpkin Seedling in Salinity. Journal of Seed Research. 7(2): 10-20. (in Persian, abstract in English)
Mousavi, S.G. and Jouyban, Z. 2012. Effect of salinity stress on germination and growth parameters of seedlings of basil (Ocimum basilicum L.). Technical Journal of Engineering and Applied Sciences. 2(4): 84- 87.
Munns, R. 2002. Comparative physiology of salt and water stress. Plant Cell and Environment 25(2): 239-250.
Nelson, C.P. 2000. Water potential: The key to successful seed priming. Decagon Devices, Inc. 2000. AN4101- 10.
Nun, N.B., Plakhine, D., Joel, D.M. and Mayer, A.M. 2003. Changes in the activity of the alternative oxidase in orobanche seeds during conditioning and their possible physiological function. Phytochemistry. 64(1): 235-241.
Parida, A.K. and Das, A.B. 2005. Salt tolerance and salinity effects on plants: a review, Ecotoxicology and Environmental Safety. 60: 324-349.
Pedram, A., Tajbakhsh, M., Taleghani, D. and Ghiyasi, M. 2019. Effect of seed priming on sugar beet (Beta Vulgaris L.) seedlings growth under salinity stress. Journal of Suger Beet. 35(1): 35-62.
Rajjou, L., Duval, M., Gallardo, K., Catusse, J., Bally, J. and Job, C. 2012. Seed Germination and Vigor. Annual Review of Plant Biology. 63: 507–33.
Rashid, A., Hollington, P.A., Harris, D. and Khan, P. 2006. On-farm seed priming for barley on normal, saline and saline–sodic soils in North West Frontier Province, Pakistan. European journal of agronomy. 24(3): 276-281.
Reggiani, R., Bozo, S. and Bertani, A. 1995. Effect of salinity on early seedling growth of seeds of three wheat (Triticum aestivum L.) Cultivars. Can. J. Plant Sci. 75: 175-177.
Rostami, Gh., Moghaddam, M., Narimani, R. and Mehdizadeh, L. 2018. The effect of different priming treatments on germination, morphophysiological, and biochemical indices and salt tolerance of basil (Ocimum basilicum L. cv. Keshkeni Levelou). Journal of Environmental Stresses in Crop Sciences. 11(4): 1107-1123.
Saadatian, B., Ahmadvand, G. and Soleimani, F. 2012. Effect of seed priming on germination traits of Satureja hortensis under drought and salinity stress. Journal of Seed Science and Technology. 2(2): 33-44. [In Persian with English summary].
Saadatian, B., Ahmadvand, G. and Soletmani, F. 2012. Effect of Seed Priming on Summer Savory (Satureja Hortensis) Germination Characteristic Under Drought and Salinity Stress. Seed Research (Journal of Seed Science and Technology). 2(2): 35-44.
Schabes, F.I. and Sigstad, E.E. 2008. Calorimetric studies of quinoa (Chenopodium quinoa Willd.). Seed Science. 13: 121-138.
Shah Rajabian, M.H. and Moradi, K. 2009. The effect of hydropriming time on tomato seed germination percent and seedling early growth in salinity stress. Agricultural bulletin. Islamic Azad University, Takestan unit. 1(3): 26-32. (In Persian, abstract in English)
Sheikhi, M.R. and Amini Deheghi, M. 2018. The effect of priming on some physiological traits of cumin medicinal plant (Cuminum Cyminum L) of Sabzevar massif under salt stress. The 6th Scientific Congress on the Development and Promotion of Agricultural Sciences and Natural Resources in Iran.
Shivankar, R.S., Deore, D.B. and Zode, N.G. 2003. Effect of pre-sowing seed treatment on establishment and seed yield of unflower. J. Oilseeds Res.20, 299–300.
Singh, B. and Ushu, K. 2003. Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regulators. 39(2): 137-141.
Singh, B.G. and Rao, G. 1993. Effect of chemical soaking of sunflower (Helianthus annuus L.) seed on vigour index. Indian J. Agric. Sci. 63: 232–233.
Soltani, A., Gholipoor, M. and Zeinali, M.E. 2006. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environmental and Experimental Botany. 55: 195-200.
Srivastava, A.K., Suprasanna, P., Srivastava, S.D. and Souza, S.F. 2010. Thiourea mediated regulation in the expression profile of aquaporins and its impact on water homeostasis under salinity stress in Brassica juncea roots. Plant Science. 178: 517–522.
Tajlil, A.H., Pazoki, A. and Eradatmand Asli, D. 2014. Effects of seed priming by mannitol and zinc sulfate on biochemical parameters and seed germination of chickpea. International Journal of Farming and Allied Sciences. 3: 294-298.
Yagmur, M. and Kaydan, D. 2008. Alleviation of osmotic stress of water and salt in germination and seedling growth of triticale with seed priming treatments. African Journal of Biotechnology. 7 (13): 2156-2162.