The effect of temperatur and seed moistuer content on seed germination and seedling growth characteristics of Borage (Borago officinalis L.)

Subject Areas :

Farzane Bagheri Mejdar ¹ , rasoul fakhari ² , Parisa Sheikhzadeh ³ , Nasser Zare ⁴ , Mahrokh Bolandi ⁵

1 - Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural resources, University of Mohaghegh Ardabili, Ardabil, Iran
2 - Plant Protection Research Department, Ardabil Agricultural and Natural Resources Research and Education Center, AREEO, Ardabil, Iran
3 - Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural resources, University of Mohaghegh Ardabili, Ardabil, Iran
4 - Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural resources, University of Mohaghegh Ardabili, Ardabil, Iran
5 - Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural resources, University of Mohaghegh Ardabili, Ardabil, Iran

Received: 2024-02-01 Accepted : 2024-05-30 Published : 2024-09-10

Keywords: Antioxidant enzymes, Borago officinalis, Germination, Length of seedling,

Abstract :

This research was carried out to investigate the effect of seed storage temperature and moisture content on germination characteristics, growth and biochemical characteristics of Borago officinalis seedlings, In factorial arrangement in a completely randomized design with 4 replications. The treatments included five levels of seed moisture (5, 10, 15, 20, 25%) and eight temperature levels (10, 15, 20, 25, 30, 35, 40 and 45 degrees Celsius) and 12 storage levels (7, 14, 21, 28, 35, 42, 49, 56, 63, 70, 77 and 84 day). The results showed that with increase in storage time, percentage and seed germination rate, length decreased, and also the abnormal seedlings, amount of proline seedlings and activity of peroxidase enzymes were increased. The highest germination and seedling growth were obtained in the control treatment and 7 days after storage with 10% seed moisture after 7 days of storage. The lowest germination percentage was seen 84 days after storage with %15 seed moisture at 15°C. The maximum length of seedlings was obtained at 25°C temperature and 20% humidity with 7 days of storage. The results showed that at the temperature of 25 degrees, the peroxidase enzyme activity was the highest at the beginning of the storage period (28 days of storage), but with the continued influence of adverse conditions during storage, the enzyme activity decreased significantly. The results showed that the lowest amount of proline was obtained at a temperature of 15°C with a humidity level of 25% and at the 28th day of storage. Based on the results, with increasing storage time, the moisture content of seeds and the storage temperature of the quality of Borago officinalis seedlings are reduced. Since these seeds contain oil, it should be considered to be sufficient to store, survive and maintain its maximum quality.

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