Investigating the effect of growth regulators on callusing and Somatic Embryogenesis of of Pekan variety walnut under in vitro conditions

Subject Areas : Sustainable production technologies

mohammad dali ¹ , mohammad motamedi ² , Shahab Sada ³

1 - 1- Graduated from the Department of Plant Production and Genetics, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.
2 - 2- Department of Plant Production and Genetics, Shoushtar Branch, Islamic Azad University, Shoushtar, Iran
3 - Plant Breeding Department, Islamic Azad University, Ahwaz, Iran

Received: 2024-03-01 Accepted : 2024-04-03 Published : 2024-06-19

Keywords: American walnut, embryogenesis, callus, tissue culture,

Abstract :

Walnut (Juglans regia L.) is a plant with a high economic value, and the use of micro propagation techniques will be very effective in the vegetative propagation of uniform superior cultivars and genotypes of walnut. In order to optimize walnut tissue culture, in this research, fresh branches of Pekan variety walnut trees were separated and transferred to the laboratory. For sterilization, callus formation and somatic embryogenesis, three experiments were performed with different treatments. Six sterilization treatments in a completely randomized design with 4 replications were considered for this research. In order to obtain callus, DKW culture medium was considered as the basic culture medium. 8 treatments of callus formation were investigated in a completely randomized design in 3 replications. In order to achieve indirect somatic embryogenesis, different combinations of TDZ and NAA were investigated in a completely randomized design in 4 replications. The results of analysis of variance of sterilization data indicated the existence of a significant difference between the mentioned treatments. The results of sterilization treatments showed 20% sodium hypochlorite for 5 minutes along with 70% alcohol for 15 seconds as the best result for sterilization of explants. DKW medium supplemented with 3 mg/L 2,4-D and 0.5 mg/L NAA gave the best result in callus formation. TDZ in the amount of 6 μM along with NAA in the amount of 0.2 μM had the best results in the embryogenesis of the resulting callus

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