Genetic diversity of some wild and cultivated barleys regarding their reaction to powdery mildew using ISSR and RAPD markers
Subject Areas : دو فصلنامه تحقیقات بیماریهای گیاهی
M. Ahmadi
1
(دانش آموخته کارشناسی، اصلاح نباتات، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران)
A. Fazeli
2
(استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران)
A. Arminian
3
(استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران)
Keywords: Resistance to disease, effective allele, ISSR and RAPD,
Abstract :
Genetic diversity of 34 wild and cultivated barley cultivars along with susceptible cultivar namely Afzal was evaluated regarding their sensitivity and resistance to powdery mildew in a randomly complete design with three replications in greenhouse of Ilam University. Plants were inoculated at anthesis stage with Blumeria graminis f.sp. hordei. When 16 ISSR and 6 RAPD primers were used, 125 and 32 alleles were amplified where 99.27% and 100% of the alleles had polymorphism, respectively. Average expected and observed heterogeneity and average Shanon index in ISSR were calculated to be 0.32, 0.25 and 0.35 and for RAPD were 0.28, 0.31 and 0.42. Cluster analysis by UPGMA method categorized genotypes in five clusters of which groups 1 and 2 were resistant to semi-resistant, group 3 as susceptible, and also group 4 and 5 were moderately susceptible to susceptible ones, respectively.In general, our results indicated that using molecular markers such as RAPD and ISSR can differentiate genotypes into resistant or susceptible groups that would help researchers in breeding programs.
Dizkirici A, Elif Guren H, Onde S, Ternel F, Akar T, Budak, H and Kaya, Z. 2008. Microsatellite (SSR) variation in barley germplasm and its potential use for marker assisted selection in scald resistance breeding. International Journal of Integrative Biology 4: 1–9.
Doyle JJ and Doyle JL. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.
Hiura U. 1962. Hybridization between varieties of Erysiph graminis. Phytopathology 52: 664–666.
Hou YC, Yan ZH, Wei YM and Zheng YL. 2005. Genetic diversity in barley from west China based on RAPD and ISSR analysis. Barley Genetics Newsletter 35: 9–22.
Jørgensen JH. 1988. Erysiphe graminis, powdery mildew of cereals and grasses. Advances in Plant Pathology 6:137–157
Khush GS. 1999. Green revolution: preparing for the 21st Century. Genome. 42:646–655.
Kuznetsova TE and Kalyadina TT. 1987. Study of resistance to powdery mildew in winter barley. Interrirovannaga-Zashchita-Zernovykh-Kulture to-Vreditelei-i-boleznei-pri-intensivnoc-tekhnologi-Vozdelyveniya 5–99.
Mains EB and Dietz SM. 1930. Physiological forms of barley powdery mildew, Erysiphe graminis hordei Marchal. Phytopathology 20: 22–239.
McDermott JM, Brandle U, Dutty F, Haemmerli UA, Keller S, Muhher, KE and Wolf MS. 1994. Genetic variation in powdery mildew of Barley: Development of RAPD, SCAR and VNTR Markers. Phytopathology 84: 1316–1321.
Moldovan V, Moldovan M and Kadar R. 2005. Assessment of winter wheat cultivars for resistance to Fusarium head blight. Annual Wheat Newsletter 51: 97–98.
Moseman JC. 1955. Source of resistance to powdery mildew of barley. Plant Disease Reporter 39: 967–972.
Ovesna J, Kucera L, Bockova R and Holubec V. 2002. Characterisation of powdery mildew resistance donors within Triticum boeoticum accessions using RAPDs.Plant Breeding38: 117–124.
Paillard S, Goldringer I, Enjalbert J, Doussinault G and Brabant P. 2000a. Evolution of resistance against powdery mildew in winter wheat populations conducted under dynamic management. I—is specific seedling resistance selected? Theoretical and Applied Genetics 101: 449–456.
Paillard S, Goldringer I, Enjalbert J, Trottet M, David J and Brabant P. 2000b. Evolution of resistance against powdery mildew in winter wheat populations conducted under dynamic management. II—adult resistance. Theoretical and Applied Genetics 101: 457–462.
Patpour M, Dehghan MA and Afshari A. 2006. Reaction of some dryland barley advanced lines to powdery mildew (Blumeria graminis f. sp. Hordei., Em. Marchal. Seed and Plant Journal 22 (4): 431–441.
Psraklu S, Soltanlu H, Ramezanpoor SS, Nasrollah Nejad Qomi AA, Kalateh Arabi M and Kia S. 2013. Genetic analysis of powdery mildew resistance in some barely lines. Journal of Plant Production 20(3): 49–70.
Saari EE and Prescott JM. 1975. A scale for appraising the foliar intensity of wheat disease. Plant Disease Reporter 59: 377–380.
Ward DJ. 1953. Disease and agronomic notes records on the world collection of barley grown at Bogota, Columbia in 1953. World Collection of Barley. Data Complication No. 4. United States Department of Agriculture. 148 p.
Ward DJ. 1957. Disease and agronomic data on 431 Manchurian barleys grown at five locations in the north center US and Manitoba, Canada, in 1956. World Collection of Barley Data Complication No. 5. United States Department of Agriculture. 173 p.
Younis RAA, Ismail OM and Soliman SS. 2008. Identification of Sex-specific DNA Markers for Date Palm (Phoenix dactylifera L.) Using RAPD and ISSR Techniques. Research Journal of Agriculture and Biological Sciences 4: 278–284.
Žvingila D, Vaitkuniene V, Patamsts j, Leisturmaite A, StanIute M, Balciuniene L, Česniene T, Kleizaite V and Šiuksta RV. 2012. DNA polymorphism and agronomic traits of revertants from barley (Hordeum vulgare L.) mutant tw. Žemdirbystė=Agriculture 99: 139‒148.
_||_