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Manuscript ID : 2293 Visit : 66 Page: 23 - 34

Article Type: Original Research

Controlling root-knot nematode (Meloidogyne javanica) by the nematophagous fungus Arthrobotrys oligospora and salicylic acid and study of some plant defense responses

Subject Areas : دو فصلنامه تحقیقات بیماریهای گیاهی

حدیث Mostafanezhad 1 , نوازاله Sahebani 2 , Sh Nourinejhad Zarghani 3

1 - دانشجوی کارشناسی ارشد گروه گیاهپزشکی، پردیس ابوریحان، دانشگاه تهران
2 - دانشیار گروه گیاهپزشکی، پردیس ابوریحان، دانشگاه تهران
3 - استادیار گروه گیاهپزشکی، پردیس ابوریحان، دانشگاه تهران

Received: 2017-06-10 Accepted : 2017-06-10 Published : 2013-07-23

Keywords: Phenolic compounds, Meloidogyne javanica, Arthrobotrys oligospora, control, peroxidise, polyphenol oxydase, Salicylic acid,

Abstract :

In this study, the ability of nematode-trapping fungus Arthrobotrys oligospora and
salicylic acid (SA) were examined to control root-knot nematode, Meloidogyne javanica, in
the laboratory and greenhouse conditions. Also changes of polyphenol oxidase (PPO) and
peroxidase (POX) enzymes and total phenolic compounds accumulation in different
treatments were studied. In greenhouse studies, we used leaf spraying of SA (5 mM) and soil
drenching of A. oligospora (106 spore/ml) at six-leaf stage of the plants, separately and in
combination. After 50 days of inoculation, diameter of the galls, number of galls and number
of egg masses per individual plant, number of eggs per individual egg mass, root and foliage
fresh weights were recorded. The data were analyzed in completely randomized design and
subjected to analysis of Duncan’s multiple range test (p60.05). The results showed that
combined application of A. oligospora and SA were more effective compared to individual
application of them. Study of changes in PPO, POX enzymes activities and total phenolic
compounds accumulation showed that treatment with these factors caused increase in enzyme
activities and also accumulation of total phenolics which shows induction of plant defense
system.

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