• Home
  • Identification and pathogenicity of fungal agents of root and crown rot of some cucurbit crops in shahrood region

Share To

Article Url


Manuscript ID : 2291 Visit : 66 Page: 1 - 12

Article Type: Original Research

Identification and pathogenicity of fungal agents of root and crown rot of some cucurbit crops in shahrood region

Subject Areas : دو فصلنامه تحقیقات بیماریهای گیاهی

زهرا Iraqi 1 , کامران Rahnama 2 , مجتبی Mamarabadi 3 , فرخنده Ommati 4

1 - گروه گیاهپزشکی، دانشکده تولیدات گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران. بخشی از یافته های پایان نامه کارشناسی ارشد
2 - دانشیار گروه گیاهپزشکی، دانشکده تولیدات گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان
3 - استادیار دانشکده کشاورزی، دانشگاه صنعتی شاهرود
4 - مربی پژوهشی، مرکز تحقیقات کشاورزی استان سمنان (شاهرود)

Received: 2017-06-10 Accepted : 2017-06-10 Published : 2013-07-23

Keywords: Rhizoctonia solani, Cucurbits, Fusarium spp, damping off, Phytophthora spp, Shahrood, wilting,

Abstract :

The root and crown rot is one of the most important diseases of cucurbits in the world
including Iran. In order to identify the causal agents of wilting and root and crown rot of
cucurbits (watermelon, musk melon and cantaloupe) in Shahrood, investigations and
collection of diseased samples were made in various fields of Shahrood, Mayamai and
Biargomand regions during 2009-2010. After disinfection with sodium hypochlorite (1%),
pieces of infected tissues were cultured on potato dextrose agar (PDA) medium and /or pieces
of infected tissues were cultured on PDA, CMA-PARPH and CMA-PARP media without
disinfection. Isolates producing spores were purified with single spore method while isolates
which did not produce spores, were purified using hyphal tip method. For pathogenicity tests,
seedlings grown in green house were dipped in fungal spore suspension (106/ ml) by root dip
method for spore producing fungi while in the case of isolates that did not produce spores,
pathogenicity test was performed by placing small pieces of young culture of inoculum
around crown and roots of plants being tested. According to the morphological, macroscopic
and microscopic characteristics adapted from standard keys, pathogenic isolates were
identified as Fusarium solani, F. oxysporum f.sp. melonis, F. oxysporum f.sp. niveum, F.
equiseti, Phytophthora nicotianae and Rhizoctonia solani. F. oxysporum was the dominant
isolated fungus. The results also showed that F. solani and F. oxysporum could be isolated
during all growth stages of plants (seedling up to fruiting stages) and these species were
widely spread throughout the region. All of these fungi are reported for the first time from
melon fields of Shahrood.

References:


  1. Agrios GN. 2005. Plant Pathology. 5th ed. New York: Academic Press. 635 p.
    2.
  2. Aigbe SO, Fawole B and Berner DK. 1999. A cowpea seed rot disease caused by Fusarium equiseti identified in Nigeria. Plant Disease 83: 964 (Abstract).
    3.
  3. Aegerter BJ, Gordon TR and Davis RM. 2000. Occurrence and pathogenicity of fungi associated with melon root rot and vine decline in California. Plant Disease 84: 224–230.
    4.
  4. Alexopoulos GJ, Mims GW and Blackwell M. 1996. Introductory Mycology. 4th ed. London: John Wiley & Sons, Inc. 868 p.
    5.
  5. Banihashemi SZ. 2009. Reaction of cucumis melo cultivars to races of Fusarium oxysporum f. sp. melonis the cause of melon vascular wilt. Iranian Journal of Plant Pathology 46: 11–22.
    6.
  6. Beckman CH. 1987. The Nature of Wilt Diseases of Plants. St. Paul, MN: American Phytopathological Society Press. 175 p.
    7.
  7. Behrozin M and Asadi P. 1994. Report on three Fusarium species as the causal agent of the basal root rot of onion and their distribution in East Azarbaijan. Iranian Journal of Plant Pathology 3: 41–49.
    8.
  8. Behdad E. 2006. Phytopathology And Important Plant Disease In Iran. Tehran: Atr-e-Etrat Publication. 800 p.
    9.
  9. Cooke DE, Schena L and Caccipla SO. 2007. Invited review tools to detect, identify and monitor Phytophthora species in natural ecosystems. Plant Pathology 89: 13–28.
    10.
  10. 10.  Cubeta MA and Vilgalys R. 1997. Population biology of the Rhizoctonia solani complex. Phytopathology 87: 480–484.
    11.
  11. 11.  Elmer WH, Covert SF and Donnell K. 2007. Investigation of an outbreak of Fusarium foot and fruit rot of pumpkin within the United States. Plant Disease 91: 1142–1146.
    12.
  12. 12.  Erwin DC and Ribeiro OK. 1996. Phytophthora Diseases Worldwide. St. Paul, Minnesota: American Phytopathological Society Press. 563p
    13.
  13. 13.  Ershad J. 1992. Phytophthra Species in Iran. Ministry of Agriculture. Tehran: Agricultural Research Organization. 217 p.
    14.
  14. 15.  Garibaldi A, Gilardi G, Bertoldo C and Gullino ML. 2011. First report of leaf spot of rocket (Eruca sativa) caused by Fusarium equiseti in Italy. Plant Disease 95: 1315 (Abstract).
    15.
  15. 16.  Hansen HN and Smith RE. 1932. The mechanisms of variation in imperfect fungi: Botrytis cinerea. Phytopathology 37: 369–371.
    16.
  16. 17.  Jahanbakhsh V. 1998. Investigation on foot and root rot of honeydew melon in Khorasan provinces [MSc]. [Mashhad, Iran]: University of Ferdowsi Mashhad.
    17.
  17. 18.  Pivonia S, Cohen R, Kafkafi U, Ben Ze’ev IS and Katan J. 1997. Sudden wilt of melons in southern Israel: Fungal agents and relationship with plant development. Plant Disease 81: 1264–1268.
    18.
  18. 19.  Leslie JF and Summerell BA. 2006. The Fusarium Laboratory Manual. Oxford: Wiley- Blackwell. 388p.
    19.
  19. 20.  Latani S, Taheri AH and Rahnama K. 2006. Identification and pathogenicity of Fusarium species isolated from root and crown of potato in Gorgan area. Journal of Agricultural Sciences and Natural Resources 13: 115–126.
    20.
  20. 21.  Michereff SJ, Andrade DE and Junior RS. 2008. Reaction of melon genotypes to Rhizoctonia solani. Horticultura Brasileira 26: 639–643.
    21.
  21. 22.  Nelson PE, Toussoun TA and Marasas WFO. 1983. Fusarium Species-An Illustrated Manual for Identification. Pennsylvania: Pennsylvania State University Press. 193 p.
    22.
  22. 23.  Nasrollah nezhad S. 1996. An investigation on fungal root and crown rots of cucurbits in Khuzestan province. [MSc]. [Khuzestan, Iran]: University of Shahid Chamran.
    23.
  23. 24.  Ohkura M, Abawi GS, Smart CD and Hodge KT. 2009. Diversity and aggressiveness of Rhizoctonia solani and Rhizoctonia-like fungi on vegetables in New York. Plant Disease 93: 615–624.
    24.
  24. 25.  Risser G, Banihashemi Z and Davis DW. 1976. A proposed nomeclature of Fusarium oxysporum f. sp. melonis races and resistance genes in cucumis melo. Phytopathology 66: 1105–1106.
    25.
  25. 26.  Safarnezhad MR. 2004. Investigation root rot of cucurbits in sistan region. Paper presented at: 16th Iranian Plant Protection Congress; August 28- September 1; Tabriz, Iran.
    26.
  26. 27.  Sneh B, Burpee L and Ogoshi A. 1991. Identification of Rhizoctonia species. St. Paul, Minnesota: American Phytopathological Society Press. 133 p.
    27.
  27. 28.  Stamps DJ, Waterhouse GM, Newhook FJ and Hall GS. 1990. Revised tabular key to the species of Phytophthora. Mycological Papers 162: 1-28.
    28.
  28. 29.  Shekari F, Masiha S and Esmailpour B. 2006. The Physiology of Vegetable Crops. Zanjan: Zanjan University Press. 394 p.
    29.
  29. 30.  Suarez-Estrella F, Vargas-Garcia MC, Lopez MJ and Moreno J. 2004. Survival of Fusarium oxysporum f. sp. melonis on plant waste. Crop Protection 23: 127–133.
    30.
  30. 31.  Taheri M. 2010. Identification of Phytophthora species from some crops in Gorgan region [MSc]. [Gorgan, Iran]: Gorgan University of Agricalture and Natural Resource.
    31.
  31. 32.  Tomaso-Peterson M and Trevathan LE. 2004. Rhizoctonia solani AG-13 isolated from corn in Mississippi. Plant Disease 88: 908 (Abstract).
    32.
  32. 33.  Tsao PH and Guy SO. 1977. Inhibition of Mortierella and Pythium in a Phytophthora isolation medium containing hymexazol. Phytopathology 67: 796–801.
    33.
  33. 34.  Teimouri S. 2010. Identification of the most fungal agents cause muskmelon wilting and damping off in Khorasan Razavi province [MSc]. [Gorgan, Iran]: Gorgan University of Agriculture and Natural Resource.
    34.
  34. 35.  Teimuri S, Rahnama K, Hajian Shahri M and Afzali H. 2011. Identification, distribution and pathogenicity of Fusarium species isolated from root and crown of cantaloupe and melon in Khorasan Razavi province. Researches in Plant Pathology 1: 33–44.
    35.
  35. 36.  Vujanovic V, Hamel C, Yergeau E and St-Arnaud M. 2006. Biodiversity and biogeography of Fusarium species from northeastern North American asparagus fields based on microbiological and molecular approaches. Microbial Ecology 512: 242–255.
    36.
  36. 37.  Yoshimura MA, Uchida JY and Aragaki M. 1985. Etiology and control of poinsettia blight caused by Phytophthora nicotianae var. parasitica and P. drechsleri. Plant Disease 69: 511–513.
    37.
  37. 38.  Zare R and Ershad D. 1997. Fusarium species isolated from cereal in Gorgan area. Iran Journal of Plant Pathology 33: 1–14.
    38.
  38. 39.  Zuniga TL, Zitter TA, Gordon TR, Schroeder DT and Okamoto D. 1977. Characterization of pathogenic races of Fusarium oxysporum f. sp. melonis causing Fusarium wilt of melon in New York. Plant Disease 81: 592–596.
    39.

14.  Gally T, Gonzalez B and Soberoy Rojo MB. 1998. Plumele soft rot caused by Fusarium equiseti, F. oxysporum, and F. pallidoroseum on soybean seedlings in Argentina. Plant Disease 82: 1063 (Abstract).

 

_||_

Related articles

The rights to this website are owned by the Raimag Press Management System.
Copyright © 2021-2024

Home| Login| About Us| Contact Us|
[فارسی] [العربية] [fa] [ar]