Assessment and Identification of Tolerant Genotypes of Safflower (Carthamus tinctorius L.) to Salinity Stress in Germination and Seedling Stages under Laboratory Condition

Subject Areas : crop production

Khodadad Mostafavi ¹ (Department of Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran)

Received: 2010-11-02 Accepted : 2011-09-16 Published : 2011-01-08

Keywords: Safflower, Germination percentage, Logestic model, Cluster analysis,

Abstract :

Tolerance increasing to salt stress is essential need for sustainable safflower production in areas with risk of salinity. This requires new genetic sources of salt tolerant and more efficient techniques for identifying salt-tolerant germplasm. This study was conducted to access salt tolerance in primary growth stages of six safflower genotypes and finds the most beneficial salt tolerance traits in this stage. Investigation traits were germination percentage, germination speed, root length, shoot length, seedling length, seed vigor, seedling index, germination speed index and germination mean. An experiment with factorial arrangement was conducted by using a completely randomized design (CRD) with three replications in Biotechnology laboratory Islamic Azad University- Karaj Branch. Genotypes of safflower (Carthamus tinctorius L.) including KM5, KM8, KM12, KM19, KM47 and Kouse with five concentrations of NaCl (0, -0.3, -0.5, -1 and -1.5 ds/m) were treated. Analysis of variance showed that there were significant differences between genotypes, stress levels and their interaction. According to the fitted logistic model, the NaCl concentration level required for 50% reduction of seed vigor was -2 dS/m. Cluster analysis according Ward’s method, classified genotypes into three different groups. According to the results of this research, Kose was resistant cultivar at germination stage and early seedling growth.

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