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Manuscript ID : JAPAD-2011-1106 (R1) Visit : 262 Page: 59 - 72

Article Type: Original Research

In vivo murine breast cancer targeting by magnetic iron nanoparticles involved L. GG cytoplasmic fraction

Subject Areas :

Salar Mokriyani 1 (گروه میکروبیولوژی، دانشکده علوم، دانشگاه آزاد اسلامی، واحد کرج، کرج، ایران)
Naser Harzandi 2 (گروه میکروبیولوژی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی، واحد کرج، کرج، ایران)
Amir Tukmehchi 3 (گروه میکروبیولوژی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، آذربایجان غربی، ایران)
Leila Jabalameli 4 (گروه میکروبیولوژی، دانشکده علوم، دانشگاه آزاد اسلامی، واحد کرج، کرج، ایران)

Received: 2020-11-01 Accepted : 2021-10-07 Published : 2021-09-23

Keywords: nanoparticles, anti-cancer, in vivo, Drug delivery, Probiotic,

Abstract :

Inroduction & Objective: The use of chemical anti-cancer drugs frequently create serious side effects. However, probiotics are natural and treat different kinds of cancer without undesired effects. In this study, a nano delivery system was planned to transport the Lactobacillus rhamnosus GG (L. GG) cytoplasmic fraction (Cf) to cancerous tissue in the mouse model. Material and Methods: Magnetic iron nanoparticles (MINPs) were synthesized and loaded with L. GG-Cf (0, 0.312, 0.625, 1.25, 2.5 mg/ml) and administrated for three weeks to treat experimentally induced murine breast cancer in a constant magnetic field. At the end of the trial, the treating efficacy of this complex molecule was evaluated via western blotting and qPCR. Results: Results showed MINPS can deliver and accumulate the L. GG-Cf in cancer tissue, also the size and volume of the tumors were reduced. Additionally, in cancer tissues of treated mice with 2.5 mg/ml of Cf-MINPs significant induced apoptosis was seen compared to untreated (control), and our data proved that this induction may be due to the caspase-3 pathway. Conclusion: In conclusion, L. GG-Cf could treat the murine breast cancer and MINPs are a suitable candidate for drug delivery because of their safety, uniformity, and magnetic properties.  

References:


  1. An, BC., Hong ,S., Park, HJ., Kim, BK., Ahnm, JY., Ryu, Y. (2019). Anti-colorectal cancer effects of probiotic-derived p8 protein. Genes (Basel). https:// doi.10.3390/ genes 10080624.
    2.
  2. 2. Avtanski, D., Poretsky, L. (2018). Phyto-polyphenols as potential inhibitors of breast cancer metastasis. Mol Med., https:// doi.org/ 10.1186/ s 10020-018-0032-7.
    3.
  3. 3. Benyettou, F., Ocadiz Flores, JA., Ravaux, F., Rezgui, R., Jouiad, M., Nehme, SI. (2016). Mesoporous γ-Iron oxide nanoparticles for magnetically triggered release of doxorubicin and hyperthermia treatment. Chem Europ J. https://doi.org/10.1002/chem.201602956
    4.
  4. 4. Bressenot, A., Marcha,l S., Bezdetnaya, L., Garrier, J., Guillemin, F., Plénat, F. (2009). Assessment of apoptosis by immunohistochemistry to active caspase-3, active caspase-7, or cleaved PARP in monolayer cells and spheroid and subcutaneous xenografts of human carcinoma. J Histochem Cytochem. https://doi.org/10.1369/jhc.2008.952044
    5.
  5. 5. Chen, C., Nong, Z., Xie, Q., He, J., Cai, W., Tang, X. (2017). Dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione inhibits the growth and metastasis of breast carcinoma in mice. Sci Rep. , https://doi.org/10.1038/s41598-017-07162-3
    6.
  6. 6. Chen, J., Shi, M., Liu, P., Ko, A., Zhong, W., Liao, W. (2014). Reducible polyamidoamine‑magnetic iron oxide self‑assembled nanoparticles for doxorubicin Biomaterials. https:// doi.10.1016/ j.biomaterials.2013.10.057.
    7.
  7. 7. Devarajan, E., Sahin,. AA, Chen, JS., Krishnamurthy, RR., Aggarwal, N., Brun, AM. (2002). Down-regulation of caspase 3 in breast cancer: a possible mechanism for chemoresistance. Oncogene. https:// doi.org/10. 1038 /sj.onc.1206044.
    8.
  8. 8. Gao, Z., Li, Y., You, C., Sun, K., An, P., Sun, C. (2018). Iron oxide nanocarrier-mediated combination therapy of cisplatin and artemisinin for combating drug resistance through highly increased toxic reactive oxygen species generation. ACS Applied Bio Materials., https://doi.10.1021/acsabm.8b00056.
    9.
  9. 9. Gholami, L., Tafaghodi, M., Abbasi, B., Daroudi, M., Kazemi, O.R. (2019). Preparation of superparamagnetic iron oxide/doxorubicin loaded chitosan nanoparticles as a promising glioblastoma theranostic tool. J Cell Physiol., https://doi.10.1002/jcp.27019.
    10.
  10. 10. Górska, A., Przystupski, D., Niemczura, MJ., Kulbacka, J. (2019). Probiotic bacteria: a promising tool in cancer prevention and therapy. Curr Microbiol. http://doi:10.1007/s00284-019-01679-8.
    11.
  11. 11. Hassan, Z. (2019). Anti-cancer and Biotherapeutic Potentials of Probiotic Bacteria. J Cancer Sci., https://doi.10.4172/1948- 5956.1000575
    12.
  12. 12. Hilger, I., Kaiser ,WA. (2012). Iron oxide-based nanostructures for MRI and magnetic hyperthermia. Nanomedicine., https:// doi.org/10.2217/nnm.12.112
    13.
  13. 13. Iwanowycz, S., Wang, J., Hodge, J., Wang, Y., Yu, F., Fan, D. (2016). Emodin inhibits breast cancer growth by blocking the tumor-promoting feedforward loop between cancer cells and macrophages. Mol Cancer Ther. https://doi.10.1158/1535-7163.MCT-15-0987
    14.
  14. 14. Jazzara, M., Ghannam, A., Soukkarieh,. C, Murad, M. (2016). Nti-proliferative activity of ƛ-carrageenan through the induction of apoptosis in human breast cancer cells. Iran J Cancer Prev., https://doi.10.17795/ijcp-3836.
    15.
  15. 15. Kim, JY., Woo, HJ., Kim, Y., Lee, HJ. (2002). Screening for antiproliferative effects of cellular components from lactic acid bacteria against human cancer cell lines. Biotechnol Letters., https://doi.org/10.1023/A:1019875204323.
    16.
  16. 16. Kossatz S, Grandke J, Couleaud P, Latorre A, Aires A, Crosbie-Staunton K, Ludwig R, Dähring H, Ettelt V, Lazaro-Carrillo A, Calero M, Sader M, Courty, J, Volkov Y, Prina-Mello A, Villanueva A, Somoza Á, Cortajarena AL, Miranda R, Hilger I (2015) Efficient treatment of breast cancer xenografts with multifunctionalized iron oxide nanoparticles combining magnetic hyperthermia and anti-cancer drug delivery. Breast Cancer Res. https://doi.10.1186/s13058-015-0576-1.
    17.
  17. 17. Lee, JE., Lee, J., Kim, JH., Cho, N., Lee, SH., Park, SB. (2019). Characterization of the anti-cancer activity of the probiotic Bacterium Lactobacillus fermentum using 2D vs. 3D culture in colorectal cancer cells. Biomolecules., https://doi.org/10.3390/biom9100557.
    18.
  18. 18. Li, Z., Yu, Y., Wang, M., Xu, H., Han, B., Jiang, P. (2019). Anti-breast cancer activity of spg-56 from sweet potato in mcf-7 bearing mice in situ through promoting apoptosis and inhibiting metastasis. Sci Rep. https:// doi.org/10. 1038/s41598-018-29099-x.
    19.
  19. 19. Lin, PW., Nasr, TR., Berardinelli, AJ., Kumar, A., Neish, AS. (2008). The probiotic Lactobacillus GG may augment intestinal host defense by regulating apoptosis and promoting cytoprotective responses in the developing murine gut. Pediatric Research. https://doi. org/10.1203/PDR. 0b013e3181827c0f.
    20.
  20. 20. Lu, X., Zhum, Y., Bai, R., Wu, Z., Qian, W., Yang, L., Cai, R. (2019). Long-term pulmonary exposure to multi-walled carbon nano-tubes promotes breast cancer metastatic cascades. Nat Nanotechnol.,https://doi.org/10.1038/s41565-019- 0472-4.
    21.
  21. 21. Ma, EL., Choi, YJ., Choi, J., Pothoulakis, C., Rhee, SH., Im, E. (2010). The anticancer effect of probiotic Bacillus polyfermenticus on human colon cancer cells is mediated through ErbB2 and ErbB3 inhibition. Int J Cancer., https:// doi.10.1002/ijc.25011
    22.
  22. 22. National Research Council (NRC) 8th ed. Washington, USA: National Academies Press; 2011. Committee for the update of the guide for care and use of laboratory animals; pp. 11-41.
    23.
  23. 23. Ndong, C., Tate, JA., Kett, WC., Batra, J., Demidenko, E., Lewis, LD. (2015). Tumor cell targeting by iron oxide nanoparticles is dominated by different factors in vitro versus in vivo. Plo one. https://doi. org/10.1371/ journal.pone.0115636.
    24.
  24. 24. Nigjeh, SE., Yeap, SK., Nordin, N., Rahman, H., Rosli, R. (2019). In vivo anti-tumor effects of citral on 4t1 breast cancer cells via induction of apoptosis and downregulation of aldehyde dehydrogenase activity. Molecules. https://doi. org/10.3390/molecules24183241.
    25.
  25. 25. Nowak, A., Paliwoda, A., Błasiak, J. (2019). Anti-proliferative, pro-apoptotic and anti-oxidative activity of Lactobacillus and Bifidobacterium strains: A review of mechanisms and therapeutic perspectives. Crit Rev Food Sci Nutr., https://doi. 10.1080/ 10408398.2018.1494539.
    26.
  26. 26. Orlando, A., Messa, C., Linsalata, M., Cavallini, A., Russo, F. (2009). Effects of GG on proliferation and polyamine metabolism in HGC-27 human gastric and DLD-1 colonic cancer cell lines. Immunopharmacol Immunotoxicol. https://doi.10. 1080/ 08923970802443631.
    27.
  27. 27. Orlando, A., Refolo, MG., Messa, C., Amati, L., Lavermicocca, P., Guerra, V. (2012). Antiproliferative and proapoptotic effects of viable or heat-killed Lactobacillus paracasei1 and Lactobacillus rhamnosus GG in HGC-27 gastric and DLD-1 colon cell lines. Nutr Cancer,https://doi.org/10.1080/01635581.2012.717676.
    28.
  28. 28. Orlando, A., Linsalata, M., Russo, F. (2016). Antiproliferative effects on colon adenocarcinoma cells induced by co-administration of vitamin K1 and Lactobacillus rhamnosus Int J Oncol., https:// doi.10.3892/ ijo.2016.3463.
    29.
  29. 29. Pepper, SJ., Britz, ML. An. (2019). Acid up-regulated surface protein of Lactobacillus paracasei strain GCRL 46 is phylogenetically related to the secreted glucan- (GpbB) and immunoglobulin-binding (SibA) protein of pathogenic streptococci. Int J Mol Sci., https://doi.org/10.3390/ijms20071610
    30.
  30. 30. Piehler, S., Dähring, H., Grandke, J., Göring, J., Couleaud, P., Aires, A. (2020). Iron oxide nanoparticles as carriers for dox and magnetic hyperthermia after intratumoral application into breast cancer in mice: impact and future perspectives. Nanomaterials (Basel). https://doi. 10.3390/ nano10061016.
    31.
  31. 31. Sharifi, M., Hasan, A., Nanakali, NMQ., Salihi, A., Qadir, FA., Muhammad, HA. (2020) Combined chemo-magnetic field-photothermal breast cancer therapy based on porous magnetite nanospheres. Sci Rep., https://doi. org/10.1038/ s41598-020-62429-6.
    32.
  32. 32. Sharma Singh, RL., Kakkar, P. (2011). Modulation of Bax/Bcl-2 and caspases by probiotics during acetaminophen induced apoptosis in primary hepatocytes. Food Chem Toxicol. https:// doi.org/ 10.1016/j. fct.2010. 11.041.
    33.
  33. 33. Wang, Y., Wu, Y., Wang, Y., Xu, H., Mei, X., Yu, D. (2017). Antioxidant properties of probiotic bacteria. Nutrients., https:// doi.org/ 10.3390/ nu9050521.
    34.
  34. 34. Wu, H., Inoue, M. (2006). Immuno histochemical analysis for Mdm2 and p53 proteins in methylcholanthrene-induced mouse rhabdomyosarcomas. J Vet Med Sci., https:// doi.org/ 10.1292/jvms.68.427.
    35.
  35. 35. Wu, M., Huang, S. (2017). Magnetic nanoparticles in cancer diagnosis, drug delivery and treatment. Mo. Cli. Oncol., https:// doi. 10.3892/ mco.2017.1399.
    36.
  36.  Xuan, S., Wang, F., Lai, FMY., Sham, KWY., Wang, XJ., Lee, S. (2011). Synthesis of biocompatible, mesoporous Fe3O4 nano/ microspheres with large surface area for magnetic resonance imaging and therapeutic applications. ACS Appl Mater Int., https:// doi.10.1021/ am1012358.
    37.
  37. Zhou, Y., Tian, L., Zhang, YC., Guo, BF., Zhou, QW. (2014). Apoptotic effects of psiRNA-STAT3 on 4T1 breast cancer cells in vitro. Asian Pac J Cancer Prev., https:// doi.org/ 10.7314/ APJCP. 2014.15.16.6977..
    38.

 

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