Investigation of Antibacterial Activity of Magnetite, Magnetite/Zinc Oxide and Magnetite/Zinc Oxide/Chitosan on Some Bacteria Inducing Infection in Skin Wounds
Subject Areas :
1 - department of microbiology,biology research center ,zanjan branch, islamic azad university
Keywords: Infectious bacteria, zinc oxide, magnetite, Escherichia coli and Staphylococcus aureus,
Abstract :
Background and Objectives: Some bacteria cause infection in wound site that is a major challenge for healthiness and bacteria may be resistant to antibiotics. The current study was conducted to investigate antibacterial activity of magnetite, magnetite/zinc oxide and magnetite/zinc oxide/chitosan nanocomposites on some bacteria inducing infection in skin wounds. Materials and methods: In this study, magnetite, magnetite/zinc oxide and magnetite/zinc oxide/chitosan nanocomposites were prepared by help of Salvia officinalis extract and their effects were investigated via minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) on Escherichia coli, Staphylococcus aureus, and also Candida albicans fungus. Killing time curve was also investigated. Results: The results for MIC and MBC tests showed that magnetite/zinc oxide and magnetite/zinc oxide/chitosan nanocomposites showed significant effects on Escherichia coli and Staphylococcus aureus compared with magnetite nanocomposite but they did not show any significant effects on Candida albicans fungus. The results also showed that nanocomposites showed their effects in longer times compared with antibiotics. Conclusion: In sum, magnetite/zinc oxide and magnetite/zinc oxide/chitosan nanocomposites showed significant effects on Gram-positive and Gram-negative bacteria but in longer times.