The effect of intraperitoneal injection of hydroalcoholic extract of flaxseed on biochemical, hematological and liver enzymes in rats
Subject Areas :
Atefeh
Payez
1
(Department of biological science, Farhangian university, Tehran, Iran)
Keywords: Flaxseed, Rat, liver enzymes, Lactate dehydrogenase, Blood cells,
Abstract :
Background: Medicinal plants have a wide range of applications in various fields, especially in the control of microbial infections, but the discussion of their possible toxicity has caused great concern in the use of these substances in various fields of industry and medicine. This study was performed to clarify some of these ambiguities and to investigate the effect of intraperitoneal injection of flaxseed extract on biochemical, hematological, and hepatic factors in rats.Materials and Methods: In this experimental study, 15 female spiral rats were randomly divided into 5 groups. The first group was the sham group, the second group was the control group, and the third to fifth groups as experimental groups with concentrations of 200, 400, and 600 mg/ml of flaxseed extract were treated, respectively. The mice were treated for 10 days, then on the eleventh day the mice were anesthetized with ether and blood samples were taken from their hearts. Then biochemical parameters, blood, and lactate dehydrogenase, and plasma levels of liver enzymes were evaluated.Results: The results of this study did not show much change in biochemical factors except cholesterol and triglycerides which showed a significant decrease in the extract-treated groups. Blood factors did not show significant changes but increased in high concentrations of liver enzymes.Conclusion: Intraperitoneal injection of flaxseed extract has no effect on blood factors and many biochemical factors in mice, but high concentrations of the extract can affect liver enzymes.
1-حمزه ای، ح. ، علیزاده، ح. ، اجلی، م.، دولتشاهی، ف. 1392. اثر تزریق نانوذرات نقره بر سطوح پلاسمایی آنزیم های کبدی موش صحرایی ماده. فصلنامه فیزیولوژی و تکوین جانوری، شماره پیاپی 21، جلد 6، شماره 2، ص 48-41.
2-رضایی زارچی، س. ، تقوی فومنی، م. ، رضوی ششده، س. ، نگهداری، م. 1392. اثر نانوذرات نقره بر سلول های خونی در موش صحرایی نر. فصلنامه پژوهشی خون.جلد دهم، شماره دوم، 153-147.
3-رضویان، س.، صفرپور، ا.، روشنایی، ک.، یزدیان، م.، حیدریه، ن. 1389. بررسی تغییرات برخی پارامترهای بیوشیمیائی وهماتولوژیک درخون موش های صحرائی نژاد ویستار به موازات مصرف خوراکی نانوذرات نقره. مجله علمی پژوهشی دانشگاه علوم پزشکی بابل. دوره 13، شماره 1،27-22.
4-سهرابی پور، ش.، جعفری، ع.، کمالی نژاد، م.، صراف نژاد، ع.، شهرستانی، ط.، صادقی پور، ح.1390. بررسی اثر بذر کتان بر وضعیت استرس اکسیداتیو در موشهای صحرایی نابالغ مبتلا به واریکوسل. فیزیولوژی و فارماکولوژی، جلد 15 ،شماره3 ، ص 426 – 415.
5-علیزاده، ح.، شاپوری، ر.، شکری، ر.، دولتیاری، ل. 1389. بررسی اثر ضد میکروبی عصاره میوه و دانه به بر روی برخی باکتری های عامل عفونت پوستی. فصلنامه علوم زیستی دانشگاه آزاد اسلامی واحد زنجان ،شماره 1 ، جلد 4، ص 92-87.
6-نقش، ن.، مشایخ، ا.، خدادادی، سمانه. 1392. تاثیر نانوذرات نقره بر فعالیت آنزیم لاکتات دهیدروژناز و تغییرات بافت قلب در موشهای صحرایی نر نژاد ویستار. مجله دانشگاه علوم پزشکی فسا، جلد چهارم، شماره دوم ، 307-303.
7.Adolphe, J.L., Whiting, S.J., Juurlink, B.H., Thorp, L.U., Alcorn, J. (2010). Health effects with consumption of the flax lignan secoisolariciresinol diglucoside. Br J Nutr, 103;929-938.
8.Afzal, U., Butt, M. S., Ashfaq, F., Bilal, A., Suleria, H. A. R. (2020). Bioassessment of flaxseed powder and extract against hyperglycemia and hypercholesterolemia using Sprague Dawley rats. Clinical Phytoscience, 6(1); 1-13.
9.Atkins, C.G., Kevin B., Michael W. B., Robin, Turner F.B. (2017). Raman spectroscopy of blood and blood components. Applied Spectroscopy, 71( 5); 767-793.
10.Babu S., Mitchell, G.V., Wiesenfeld, P.,
Jenkins, M. Y., Gowda, H. (2000). Nutritional
and hematological impact of dietary flaxseed and defatted flaxseed meal in rats. International Journal of Food Sciences and Nutrition, 51(2);109-117.
11.Bussler, S., Mandy, V., Diana, P., Kristian, H., Theresa, B., Melanie, P., Norman, H. et al. (2018). "New pediatric percentiles of liver
enzyme serum levels (alanine aminotransferase, aspartate amino transferase, γ‐glutamyl transferase): Effects of age, sex, body mass
index, and pubertal stage. Hepatology, 68(4); 1319-1330
12.Chen, L.Y., Hui Kuan, C., Tao, B., Sheng Jin, T., Yuan, W., Zhong Lin, L., et al. (2020). Liver damage at admission is an independent prognostic factor for COVID‐19. Journal of Digestive Diseases, 21( 9); 512-518.
13.Chen, Y.L., Hitoshi, S., Nien-Shan, L., Hsiang-Chi, P., Qian, X., Suh-Ching, Y. (2020). Impacts of fish oil on the gut microbiota of rats with alcoholic liver damage. The Journal of Nutritional Biochemistry, 86; 108-116.
14.Clark, W.F., Parbtani, A., Huff, M.W. (1995). Flax seed: a potential treatment for lupus nephritis. Kidney Int, 48(2); 475-480.
15.Ebrahimi Vosta Kalaee, S., Talebi Mazraeh Shahi, A., Naseri, M. (2014). Anti-inflammation effect of alcoholic extract of linum . JBUMS, 16 (4); 50-56.
16.Hudson, A., Elizabeth, L., Ahmad, J., Almalki, A.L.R., Angela, I.C. (2018). A review of the toxicity of compounds found in herbal dietary supplements. Planta Medica, 84(9,10); 613-626.
17.Jamalzadeh, H.R., Keyvan, A., Ghomi, M., Oryan, S. (2008). An assessment of hematological and serum biochemical indices in Salmo trutta caspius. Iran Sci Fisher J, 17(3); 47-54. (Persian).
18.Kinniry, P., Amrani, Y., Vachani, A., Solomides, C.C., Arguiri, E., Workman, A. (2006). Dietary flaxseed supplementation ameliorates inflammation and oxidative tissue damage in experimental models of acute lung injury in mice. J Nutr, 136; 1545-1551.
19.Le, A., Cooper, C.R., Gouw, A.M., Dinavahi, R., Maitra, A., Deck, L.M. (2010). Inhibition of lactate dehydrogenase a induces oxidative stress and inhibits tumor progression. Proceedings of the National Academy of Sciences, 107(5); 2037-2042.
20.Lee, J.C., Bhora, F., Sun, J., Cheng, G., Arguiri, E., Solomides, C.C. (2008). Dietary flaxseed enhances antioxidant defenses and is protective in a mouse model of lung ischemia-reperfusion injury . Am J Physiol Lung Cell Mol Physiol, 294; 255- 265.
21.Liu, C., Zi-Cheng, J., Chu-Xiao, S., Hong-Guang, Z., Hong-Mei, Y., Zhen-Huai, C. (2020). Preliminary study of the relationship between novel coronavirus pneumonia and liver function damage: a multicenter study. Zhonghua gan zang bing za zhi= Zhonghua ganzangbing zazhi= Chin J Hepat, 28(2); 148-152.
22.Monroe, G.R., Albertien, M., Federico, T., Karen, J.D., Sanne, M.C.S., Johanna, C. (2019). Identification of human D lactate dehydrogenase deficiency. Nature Communications, 10(1); 1-8.
23.Poswal, F.S., Grace, R., Marion, M., Euan, M.L., Emmanuel, C. A., Vivien, R. (2019). Herbal teas and their health benefits: a scoping review. Plant Foods for Human Nutrition, 74(3); 266-276.
24.Pourjafari, F., Haghpanah, T., Sharififar, F., Nematollahi-Mahani, S.N., Afgar A., Asadi Karam, G. (2019). Protective effects of hydro-alcoholic extract of foeniculum vulgare and linum usitatissimum on ovarian follicle reserve in the first-generation mouse pups. Heliyon, 5(10); 25-40.
25.Prasad, K. (2005). Effect of chronic administration of lignan complex isolated from flaxseed on the hemopoietic system. Molecular and cellular biochemistry, 270(1); 139-145.
26.Ranjzad, M. (2008). Evaluation of flaxseed yield and yield components and oil characteristics of oil seed Family cultivars (Linum usitatissimum L.). Iran J Med Aromatic Plants, 24(3); 253-262.
27.Venkateswara, R.J. (2006). Toxic effects of novel organophosphorus insecticide (RPR-V) on certain biochemical parameters of euryhaline fish, Oreochromis mossambicus. Pesticide Biochem and Physiol, 86; 78-84.
28.Yu, Z., Hamed. S., Allison, M. D., Renwei, W., Carolyn, J. T., Chung, S.Y. (2017). Effect of green tea supplements on liver enzyme elevation: results from a randomized intervention study in the United States. Cancer Prevention Research, 10(10); 571-579.
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