Effect of Sublethal Concentrations of Diazinon in Testicular Tissue in Zebrafish (Danio rerio)
Subject Areas :معصومه درویشی مجره 1 , رقیه صفری 2
1 - دانشگاه علوم کشاورزی و منابع طبیعی گرگان
2 - دانشگاه علوم کشاورزی و منابع طبیعی گرگان
Keywords: Danio rerio, Testicular tissue, diazinon,
Abstract :
Inroduction & Objective: Diazinon is an organophosphoric pesticide used in agricultural fields. The aim of this study was to evaluate the effect of sublethal concentrations of diazinon on testicular tissue in Danio rerio. Material and Methods:For this purpose, 240 male animals with mean weight of 0.20 ± 0.05 were subjected to 0.8, 1.6 and 3.2 mg / l of diazinon and control for 30 days. Results: In histopathological study, the highest abnormality was observed in the highest dose (3.2 mg / l) and the most important abnormality was reduction in gonad size. In control group, the testicular tissue had adequate size and growth, and the cells were in the sexual maturity stage, but in 0.8 mg/ l, cellular atrophy and lose of lobular walls was observedIn 1.6 mg / l, the degeneration and atrophy of the sexual cells, destruction of shape and walls of the lobules was observed. At the highest concentration, the degeneration and atrophy of the sexual cells, destruction of shape and wall of the lobules and the decrease in the number of sex cells were observed. This decrease in the number of sexual cells results in reduction of testicular tissue size. Conclusion: These observations indicate an increase in complications and anomalies with the increase in toxin concentrations
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