Effects Of Aqueous Extract Of Glycyrrhiza glabra Root Against Lipid Dysfunction and Hepatic Tissue Changes Induced by Thioacetamide in Male Rats

Subject Areas :

Davood Moghadamnia ¹ , Moktar Mokhtari ² , Saeed Khatam Saz ³

1 - گروه زیست شناسی،واحد علوم وتحقیقات فارس،دانشگاه آزاداسلامی، فارس. ایران
2 - گروه زیست شناسی،واحد شیراز ،دانشگاه آزاد اسلامی،شیراز. ایران
3 - گروه زیست شناسی،واحدکازرون ،دانشگاه آزاد اسلامی،کازرون. ایران.

Received: 2016-04-18 Accepted : 2018-08-04 Published : 2015-04-21

Keywords: glucose, Male rat, Thioacetamide, Lipid Dysfunction, Glycyrrhiza glabra,

Abstract :

Inroduction and ObjectiveThioacetamide cause hepatocellular carcinoma and lipid dysfunction.The plant phenolic compounds such as flavonoids have a important role in the treatment of many disease. The present study was done to evaluate  effects of aqueous extract of Glycyrrhiza glabra root against lipid dysfunction and hepatic tissue changes induced by thioacetamide in male rats. Materials and Methods:35 male rats were divided into five groupsof seven:Control group.Animals of this group underwent no stress such as injection ,oral gavage and etc.Sham group.Animals of this group received150 mg/kg of thioacetamide intrapertionealy in a single dose  for 3 months.Experimental  group 1,2,3. Animals of this groups  received 100,200,300 mg/kg of aqueous extract of glycyrrhiza glabra root orally per day for 3 months and  then 150 mg/kg of thioacetamide intrapertionealy in a single dose. All animals bled after 3 months .The provided blood samples were tested for total cholesterol, LDL and HDL cholesterol,triglyceride and glucose serum levels by enzymatic methods. The pathological examination of hepatic tissue samples were done after hematoxylin - eosin stainingResultsPretreatmen with 100,300 mg/kg of  aqueous extract of  Glycyrrhiza glabra root significandecreased  the serum levels of total choesterol comparing with thioacetamide group.Pretreatmen with the aqueous extract of  Glycyrrhiza glabra root at all doses increased  the serum levels of glucose comparing with thioacetamide group but there was no significant difference. Pretreatmen with the aqueous extract of  Glycyrrhiza glabra root at all doses showed  no  significant  difference inserum levels of LDL,HDLcholesterol and triglyceride comparing with thioacetamide group. In all experimental groups the hepatic tissue changes induced by thioacetamide improved which were dose dependent.(p≤.05) Conclusion: Therefore, the result show that the protective effects of aqueous extract  of  Glycyrrhiza glabra root against Lipid dysfunction and hepatic tissue induced by thioacetamide in male rats.The protective effects of this extract can be due to the presence of flavonoids,chalcones,phytosterols compounds.