Interactions between D1 Dopaminergic Systems Of Nucleus Accumbens Shell and Glutamatergic System Of Prelimbic on Anxiety- Like Behaviors Of Wistar Male Rats
Subject Areas :Hatam Ahmadi 1 , Parvin Rostami 2 , Mohammad Reza Zarrindast 3 , Mohammad Nasehi 4 , Homa Mohseni Kokhesfahani 5
1 - دانشگاه خوارزمی( تربیت معلم )، دانشکده علوم زیستی، تهران. ایران
2 - استاد دانشگاه خوارزمی( تربیت معلم )، دانشکده علوم زیستی، تهران. ایران.
3 - استاد فارماکولوژی، دانشگاه علوم پزشکی تهران، دانشگاه تهران، گروه فارماکولوژی، تهران. ایران
4 - ستادیار فیزیولوژی جانوری دانشگاه آزاد اسلامی گروه زیست شناسی، واحد گرمسار، سمنان.ایران.
5 - دانشیار بیولوژی تکوینی دانشگاه خوارزمی( تربیت معلم )، دانشکده علوم زیستی، تهران. ایران.
Keywords: Anxiety-Like Behaviors, Prelimbic, Glutamatergic System, Accumbens Shell, Dopaminergic System,
Abstract :
Inroduction and Objective:Nucleus accumbens(NAC), prefrontal cortex(PFC) dopaminergic and glutamatergic systems are involved in regulating anxiety- like behaviors, while NAc dopaminergic system activity is mediated by glutamatergic projections, via PFC.This study has investigated the involvement of NAc shell D1 dopaminergic and prelimbic glutamatergic systems on anxiety- like behaviors.Material and Methods:Elevated plus-maze apparatus was employed to test parameters of anxiety-like behaviors After 5 days surgery of male Wistar rats in a stereotaxic frame ,Elevated plus-maze apparatus was employed for measuring parameters of anxiety-like behaviors due to intrabrain injection of drugsSKF38393(dopamine D1 receptor agonist)SCH23390(dopamine D1 receptor antagonistNMDA(receptor antagonist), D-AP7NMDA receptor antagonist on Wistar male rats.Results: Unilateral intra- prelimbic injection of NMDA (0.9 μg/μl), induced anxiolytic-like behaviors which has been blocked by D-AP7(NMDA receptor antagonist, 0.25, 0.5 and 1 μg/μl). Moreover, unilateral infusion of SKF38393 (dopamine D1 receptor agonist, 3 μg/μl) 0.25, 0.5 and 1μg/μl) into the left NAc shell induced anxiolytic-like behaviors which was blocked by SCH23390 (0.25μg/μl), while, SCH23390 (dopamine D1 receptor antagonistdid not so; Furthermore, intra-NAc shell administration of subthreshold dose of SKF38393 (1 μg/μl) potentiated the lower dose, whereas decreased the higher dose of intra-left prelimbic NMDA response. In addition, the subthreshold dose infusion of SCH23390 (0.25 μg/μl) into the left NAc shell, intra- prelimbic NMDA reduced induced anxiolytic-like behaviors. Conclusion: The results demonstrated a modulatory effect of NAC shell D1 dopaminergic system on anxiolytic-like response by injection in prelimbic NMDA.