The Effect Of Berberis vulgaris Leaf Aqueous Extract on Angiogenesis in Chick Chorioallantoic Membrane
Subject Areas :Shima Shayestehpour. 1 , Saiedeh Zafarbalanejad 2 , Khadijeh Nejad Shahrokh Abadi 3
1 - کارشناسی ارشد علوم جانوری(تکوینی)، گروه زیست شناسی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد. ایران.
2 - استادیار گروه زیست شناسی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد.ایران
3 - استادیار گروه زیست شناسی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد.ایران
Keywords: angiogenesis, Berberis vulgaris, Chorioallantoic Membrane (CAM),
Abstract :
Inroduction and Objective: Angiogenesis, the formation of new blood vessels, is a complex activity which is needed for embryogenesis and other physiological processes. However, in many pathological conditions such as solid tumor progression, the disease appears to be associated with persistent up-regulated angiogenesis. In this research, the effect of Berberis vulgaris leaf aqueous extract on angiogenesis in chick chorioallantoic membrane was examined.Material and Methods:50 Ross fertilized eggs were randomly divided in 5 groups as following: control, sham-exposed and experimental groups; treated with Berberis vulgaris leaf aqueous extract at different concentration. On day 2 a window was opened on eggs and on the day 8 a gelatin sponge was placed on chorioallantoic membrane (CAM) which was soaked with 10 microliters of PBS in sham-exposed group and in experimental groups with 10 microliters of the extract at concentrations of 100, 200 and 500 mg/ml were treated. On day 12 CAM in all cases were photographed. The number and length of vessels were measured by using Image J software, the data were analyzed by t and ANOVA (pResults: The comparison of average number in experimental groups with control group showed a significant decrease in experimental groups 2 and 3. The comparison of average length in experimental groups with control group showed a significant decrease in all cases.Conclusion:According to the results of this study Berberis vulgaris leaf aqueous extract can have an inhibitory effect on angiogenesis in CAM which was seen as a decrease of vessels number and length in treated area.