Effects of intracerebroventricular injection of ketorolac and methylprednisolone on antioxidant activity in brain tissue of epileptic rats
Subject Areas : Experimental physiology and pathology
zohreh fazlelahi
1
,
Jahangir Kaboutari
2
*
,
Morteza Zendehdel
3
,
Negar Panahi
4
1 - Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 -
3 - Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4 - Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
Keywords: Oxidative stress, Epilepsy, Ketorolac, Methylprednisolone, Rat,
Abstract :
Background & Aim: Epilepsy is a pervasive brain disorder and oxidative stress is considered as one of the most important mechanisms involved in epilepsy. Rather than treating epilepsy, current drugs merely control seizure symptoms and are ineffective in 30 percent of patients. Therefore, identifying more effective medications for the treatment of epilepsy is of great importance. In this regard, the present study was carried out with the aim of investigating the effects of intracerebroventricular (ICV) administration of ketorolac and methylprednisolone on the oxidative stress indices of the brain tissue of epileptic rats.
Materials & methods: In the current study, 48 female rats were randomly divided into 8 experimental groups to perform two experiments. In the first experiment, normal saline and ketorolac in doses of 7.5, 15, and 30 μg and in the second experiment, normal saline and methylprednisolone in doses of 0.15, 0.3, and 0.6 μg were injected ICV, respectively, and acute epilepsy was also induced by intraperitoneal administration of pentylenetetrazole in rats. Half an hour after the injections, the rats were euthanized, and after separating the brain, the indicators of oxidative stress, total antioxidant capacity, malondialdehyde, and nitric oxide levels were measured in hippocampal homogenous tissue.
Results: Based on the findings, the injection of ketorolac and methylprednisolone in a dosedependent manner caused a significant increase in the total antioxidant capacity of the treatment groups compared to the control group (P<0.05). Also, the administration of different doses of both drugs significantly reduced the level of malondialdehyde and nitric oxide compared to the control group (P<0.05).
Conclusion: The results of this study showed that ketorolac and methylprednisolone probably have antioxidant effects and can be further investigated as potential treatment options for epilepsy and other diseases related to oxidative stress.
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