Investigating the effects of silver nano particles on fungi flora of seeds of Chamran wheat cultivar

Subject Areas : Plant Protection

Mohsen Ghamari ¹ , Masoud Latifian ² , Mansour Farbod ³ , Nazanin Amir Bakhtiar ⁴

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Received: 2011-05-09 Accepted : 2011-09-10 Published : 2011-11-22

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Abstract :

This study was carried out in order to investigate the possibility of  controlling seed-born disease of wheat Chamran cultivar by  nano silver seed coating and indicate relevant concentration of  nano silver on fungal seed-born of wheat at Khouzestan-Ramin Agricultural and Natural Resources University. Experiment was conducted in the form of completely randomized design with four replications and six treatments. Treatments included one level of disinfection by 2.5% sodium hypochlorite and five levels of seed coating by different concentration  of silver nano particle (S1 = 1.30, S2 = 4, S3 = 6.70, S4 = 9.3 and S5 = 2666 mg of silver nitrate solution). Results showed that there was a significant difference among disinfection levels (P≤0.01) for disease index. In comparison with sodium hypochlorite, the Disease Index was declined about 50% by S1 treatment. The most favorable effect on decreasing Disease Index was observed with S5 treatment where it dropped up to 75%. According to results the mean of mortal concentration of nano silver on dominant species (Aspergillus sp.,fusarium graminiarum, Penicilium sp ., Torula sp.,  Alternaria alternaria) was calculated using Fini method in order to approach the finest concentration. The maximum LC50 was obtained for Torula sp. 1875.6 ml/lit and the minimum 0.02 ml/lit was for Aspergillus sp. and Alternaria alternaria. Regarding to this point that fungal seed-borne are active on seed bed in the form of a band, for approach to efficient disinfection it is essential to use the maximum needed concentration. 

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