Genetic diversity in varieties and lines of common bean (Phaseolus vulgaris) using RAPD marker

Subject Areas : Plant Protection

Alireza Eftekharian Jahromi ¹ , Mohammad Reza Naghavi ² , Amir Mousavi ³ , Ali Niazi ⁴

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Received: 2013-04-26 Accepted : 2015-01-02 Published : 2014-11-22

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Abstract :

Genetic variation in 75 common bean genotypes from three regions (Markazi province, Lorestan province, CIAT center) was assessed using RAPD markers. PCR analysis was performed on genomic DNA using 6 primer pairs. PCR products were detected agarose gel. The proportion of polymorphism with an average of 58% varied from 38 for OPO3 to 78 for OPBD20. The average of polymorphism information content was 0.26. OPM9 and OPO10 primers had the highest and lowest value (0.35 and 0.17). Nei’s gene diversity and Shannon s information index were 0.18 and 0.14. Genetic similarity was estimated using the Nei coefficient, and dendrogram was constructed using the UPGMA method. Generally, results showed the similarity of genetic structure between the three populations of common bean.  

References:

Agrama, H. A and Tuinstra, M. R. 2003. Phylogenic diversity and relationships among sorghum accessions using SSRs and RAPDs. African Journal Biotechnology, 2: 334- 340.

Anderson, J, A. and J. E. Church. 1993. Optimizing parental selection for genetic linkage map. Genome 36(1) 181-188.

 Beebe, S. Skroch, P. W. Tohme, J. Duque, M. C. Pedraza, F and Nienhus, J. 2000. Structure of genetic diversity among bean landraces of Middle American origin based on correspondence analysis of RAPD. Crop Science, 40: 264-273.

Braithwaite, K. S. and Manners, J. M. 1994. DNA markers reveal hybrids between two diverse background genotypes in Australian collections. Australian Journal of Botany: 42: 255-267.

Chtourou-Ghorbel, N., Lauga, B., Brahim, N., Combes, D. and Marrakchi. M. 2002. Genetic variation analysis in the genus Lathyrus using RAPD markers. Genetic Resources and Crop Evolution, 49: 363-370.

Danylchenko, O and Sorochinsky, B. 2005. Use of RAPD assay for the detection of mutation changes in plant DNA induced by UV-B and g-rays. BMC Plant Biology, 5 (Suppl. 1), S9.

Duran, L. Blair, M.W. 2005. Morphological and molecular characterization of common bean landraces and cultivars from the Caribbean. Crop Science.45:1320-1328

Grisi, M.C.M., Blair, M.W. and Gepts, P. 2007. Genetic mapping of a new set of microsatellite markers in a reference common bean population BAT93 × Jalo EEP558. Genetic and Molecular Research, 6:691-706

Martins, S. R., Venses, F. J., Saenz, L. E., Barroso, M. R. and Carnide, V. 2006. RAPD analysis of genetic diversity among and within Portuguese landraces of common white bean (Phaseolus vulgaris L). Scientia Horticulturae108:133-142.

Matus, I. A. and Hayes, P. M. 2002. Genetic diversity in three groups of barley germplasm assessed by simple sequence repeats. Genome, 45: 1095- 1106.

Prasad, M., Varsheny, R. K. and Roy J. K. 2000. The use of microsatellite for detection DNA polymorphism, genotype identification and genetic diversity in wheat. Theoretical and Applied Genetics, 100: 584-52.

Roder, M. S. and Korzun, V. 1998. A microsatellite map of wheat. Genetics, 149: 2007-2023.

Saraladevi, M. and Selvaraju, A. 2008. Efficiency of RAPD and ISSR markers system in accessing genetic variation of bean landraces. Electronic Journal of Biotechnology, 11 (3):1-10.

Yeh, F. C. and Yang, R. C. 1999. POPGENE, the user-friendly Shareware for Population Genetic Analysis. Molecular Biology and Biotechnology Center, University of Alberta, Canada.

Zhang, X. Y., Blair, M. W and Wang, S. 2008. Genetic diversity of Chinese common bean (Phaseolus vulgaris L.) landraces assessed with simple sequence repeat markers. Theoretical and Applied Genetics, 117(4): 629- 640.