Efficacy of some fungal and bacterial bioagents against Fusarium oxysporum f.sp. ciceri on chickpea

Subject Areas : Plant Protection

Hesamedin Ramezani ¹

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Received: 2016-10-04 Accepted : 2008-12-30 Published : 2008-01-01

Keywords:

Abstract :

The efficacy of four fungal bioagents viz., Trichoderma hamatum, Trichoderma harzianum, Trichoderma viride, Gliocladium virens and two bacterial bioagents namely Pseudomonas fluorescens and Bacillus subtilis were evaluated against the chickpea vascular wilt pathogen, Fusarium oxysporum f.sp. ciceri in vitro condition using Dual Culture Technique. Among the fungal bioagents, T. harzianum produced the maximum inhibition zone of 17 mm compared to the minimum of 7 mm by T. hamatum. There was no significant difference between the inhibition zones produced by P. fluorescens and B. subtilis. Soil application of talc- based formulation of T. harzianum, P. fluorescens and G. virens effectively controlled the vascular wilt of chickpea under field condition.  

References:

Agrios, G.N. 2000. Significance of plant disease, pp. 25-37, In: Agrios, G.N. (Ed.), Plant Pathology. Academic Press, London.

Benhamou, N. & Chet, I. 1993. Hyphal interactions between Trichoderma harzianum and Rhizoctonia solani. Ultra structure and gold cytochemistry of mycoparasitic process. Phytopathology, 83(10): 1062-1071.   

Butler, E.J. 1918. Fungi and Diseases in Plants. Thacker Spink Co., Culcutta, India.

Chand, H., Chhabra, M.L. & Jalali, B.L. 1991. Promising biocontrol agents for the control of chickpea wilt. Indian Phytopathology, 46: 36-39.

Chet, I. & Baker, R. 1981. Isolation and biocontrol potential of Trichoderma hamatum from soil naturally suppressive of Rhizoctonia solani under field condition. Phytopathology, 71(3): 286-290.

Coley- Smith, J.R., Ridout, C.J., Mitchell, C.M. & Lynch, J.M. 1991. Control of bottom-rot disease of lettuce (Rhizoctonia solani) in vivo condition by using preparation of Trichoderma viride and T. harzianum or tolclofosmethyl. Plant Pathology, 40: 359-366.  

Dennis, C. & Webster, J. 1971. Antagonistic properties of species-groups of Trichoderma III. Hyphal interaction. Transactions of the British Mycological Society, 57: 363-369.

Duncan, D.B., 1955. Multiple range and multiple F-tests. Biometrics, (2-4): 1- 42.

Jeyarajan, R. & Ramaksirhnan, R. 1991. Efficacy of Trichoderma formulation against root rot disease of grain legumes. Petria, 1: 137-138.

Hadar, Y., Chet, I. & Heins, Y. 1979. Biological control of Rhizoctonia solani damping off with wheat bran culture of Trichoderma harzianum. Phytopathology, 69: 64-68.

Hayes, W.J. & Laws, E.R. 1991. Handbook of Pesticide Toxicology. Vol. 1. Academic Press Inc., New Delhi.

Kata, J. 2000. Physical and cultural methods for the management of soil borne pathogens. Crop Protection, 19: 725-731.

Kim, H.K. & Roh, M.J. 1987. Isolation, identification and evaluation of biocontrol potential of rhizosphere antagonists to Rhizoctonia solani. Korean Journal of Plant Protection, 26: 26-87.

Lifshitz, R., Windham, M.I. & Baker, R. 1986. Mechanism of biological control of pre-emergence damping off of pea by seed treatment with Trichoderma species. Phytopathology, 76(7): 720-725.

Mathew, K.A. & Gupta, S.K. 1998. Biological control of root rot of french bean caused by Rhizoctonia solani. Journal of Mycological Plant Pathology, 28: 202-205.

Papavizas, G.C. & Lewis, J.A., 1989. Effect of Gliocladium and Trichoderma on damping off  and blight of snapbean caused by Sclerotium rolfsii in green house. Plant Pathology, 38: 277-286.

Raguchander, T., Rajappan, K. & Samiappan, R., 1998. Influence of biocontrol agents and organic amendment on soybean root rot. International Journal of Tropical Agriculture, 35: 23-29.

Rajappan, K. & Ramaraj, B. 1999. Evaluation of fungal and bacterial antagonists against Fusarium moniliforme causing wilt of cauliflower. Annals of Plant Protection Society, 7(2): 205-207.

Sharma, P.D.1996. Plant Pathology. Rastogi Publ., Meerut, India.

Singh, G., Kapoor, S., Gill, A.S. & Singh, K. 1986. Chickpea varieties resistant to Fusarium wilt and food-rot. Indian Journal of Agricultural Sciences, 56(5): 344-346.

Tu, J.C. 1991. Comparison of the efficacy of Gliocladium virens and Bacillus subtilis in the control of seed rot and root rot navy beans. Mededelingen Van de Faculteit Landbourn Weteschappen Rijks Universiteit Gent, 56: 229-234.

Vidhyasekaran, P. & Muthamilan, M. 1995. Development of formulations of Pseudomonas fluorescens for control of chickpea wilt. Plant Disease, 79: 782-786.

Vidhyasekaran, P., Sethuraman, K., Rajappan, K. & Vasumathi, K. 1997. Powder formulation of Pseudomonas fluorescens to control pigeon pea wilt. Biological Control, 8: 166-171.