Evaluation of biocontrol potential of some Trichoderma and Bacillus species against the causal agent of Fusarium wilt of melons
Subject Areas : Plant ProtectionZaynolabedin Nurozei 1 , Kamran Rahnama 2 , Hojjatollah Rabbani nasab 3 , Misam Taqinasab 4
1 - Department of Plant Protection, College of Crop Sciences, University of Gorgan, Iran
2 - Department of Plant Protection, College of Crop Sciences, University of Gorgan, Iran
3 - Agricultural and natural resource research and education center of Golestan
4 - Department of Plant Protection, College of Crop Sciences, University of Gorgan, Iran
Keywords:
Abstract :
Three isolates of Trichoderma spp. and two isolates of bacillus bacterial were evaluated in biological control of Fusarium oxysporum f. sp melonis the laboratory and greenhouse condition. Dual culture, antimicrobial metabolites, and volatile metabolites were in invitro assay. Fungal pathogen colony area was recorded, compared with control and induced inhibition of growth was determined. The experiment displayed the antagonistic ability using dual culture simultaneous and dual culture un-simultaneous assays against fungal pathogen. Results showed that Trichoderma harzianum 6 whit 70% and T. viride10 whit 52.9 highest percentage of growth inhibition. Results of dual culture highest percentage of growth inhibition about 30.5 percent of isolates whit the Bacillus spp62 percent growth inhibit the growth factor. The volatile compounds Bacillus spp15 whit 12.37 percent of the precipitation was prevented from growing. In the treatment Bacillus spp62 and mixed two spice tin treatments at Trichoderma 14g1 respectively 84.93 and 84.95 were showed most effects on length growth of stem but most effects of increased fresh weight from up ground organ of plant, was mixed treatment as spices T. longibrachiatum and bacteria Bacillus spp.15. Most effects on root fresh weight were showed by Trichoderma harzianum. Most effect on stem dry weight T. viridae with T. harzianum. Most effects on increased dry weight were showed by Bacillus spp.15and T. harzianum. Results of the experiment show treatments have significantly different activity pathogen. Test average correspond were meant significant at 1% level by LSD test.
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