Evaluation of fatality potential of fungus Metarhizium anisopliae against the termite Microcerotermes diversus (Isoptera : Termitidae)

Subject Areas : Plant Protection

Amir Cheraghi ¹ , Behzad Habibpour ²

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Received: 2012-03-01 Accepted : 2012-12-08 Published : 2011-05-22

Keywords:

Abstract :

Microcerotermes diversus (Iso.: Termitidae) is one of the most destructive termites in Khuzestan province (Iran). In this study, ability of pathogenicity of the fungus Metarhizium anisopliae strain of Saravan (DEMI 001) against termite M. diversus was investigated in choice and no- choice bioassay trials by smeary and immersion methods. Results showed that concentration and time of fatality in the smeary method was higher than the immersion method. The lowest level of LC50 and LT50, it related to bioassay test no- choice with immersion method. Those level were 3.1 × 104 spore Ml-1 and 1.02 day, respectively. The feeding of treated and untreated filter paper in choice test showed that fungal conidial suspension in concentrations used in this study had no repellency effect against the target termite. Overall, this study showed that the fatality rate of fungal pathogen. M. anisopliae against termite M. diversus was associated with the fungus population.  

References:

Bayon, I.L., Ansard, D., Brunet, C., Girardi, S. & Paulmier, I. 2000. Biocontrol of Reticulitermes santonensis by entomopathogenic fungi improvement of the contamination process. The International Research Group on Wood Protection, IRG/WP/DOC 00-10359.

Chouvenc, T., Su, N.Y. & Robert, A. 2009. Susceptibility of seven termite species (Isoptera) to the entomopathogenic fungus Metarhizium anisopliae. Sociobiology, 54(3): 723-748.

Edwards, R. & Mill, A.E. 1986. Termites in buildings: Their biology and control. Rentokil Limited, London.

Habibpour, B. 1994. Termites (Isoptera) fauna, economic importance and their biology in Khuzestan, (Iran). M.Sc. Thesis, College of Agriculture, Shahid Chamran University of Ahvaz, Iran.

Habibpour, B. 2006. Laboratory and field evaluation of bait-toxicants for suppression of subterranean termite populations in Ahvaz. Ph. D. Thesis, College of Agriculture, Shahid Chamran University of Ahvaz, Iran.

Habibpour, B., Cheraghi, A. & Mossadegh, M.S. 2011. Evaluation of cellulose substrates treated with Metarhizium anisopliae (Metschnikoff) Sorokin as a biological control agent against the termite Microcerotermes diversus Silvestri (Isoptera: Termitidae). Journal of Entomological & Acarological Research, 43(2): 269-275.

Hoe, P.K., Bong, C.F.J., Jugah, K. & Rajan, A. 2009. Evaluation of Metarhizium anisopliae var. anisopliae (Deuteromycotina: Hyphomycete) isolates and their effects on subterranean termite Coptotermes curvignathus (Isoptera: Rhinotermitidae). American Journal of Agricultural & Biological Science, 4(4): 289-297.

Krutmuang, P. & Mekchay, S. 2005. Pathogenicity of entomopathogenic fungi Metarhizium anisopliae against termites. Conference on International Agricultural Research for Development,11-13 October, Tropentag.

Logan, J.W.M. & El Bakri, A. 1990. Termite damage to date palms (Phoenix dactylifera L.) in Northern Sudan with particular reference to the Dongola District. Tropical Science, 30: 95-108.

Mburu, D.M., Ochola, L., Maniania, N.K., Njagi, P.G.N., Gitonga, L.M., Ndungu, M.W., Wanjoya, A.K. & Hassanali, A. 2009. Relationship between virulence and repellency of entomopathogenic isolates of Metarhizium anisopliae and Beauveria bassiana to the termite Macrotermes michaelseni. Journal of Insect Physiology, 55(9): 1-7.

Tajick Ghanbalani, M.A., Asgharzadeh, A., Hadizadeh, A.R. & Mohammadi Sharif, M. 2009. A quick method for Metarhizium anisopliae isolation from cultural soils. American Journal of Agriculture & Biological Science, 4(2): 152-155.

Verma, M., Sharma, S. & Prasad, R. 2009. Biological alternatives for termite control: A review. International Biodeterioration & Biodegradation, 63: 959-972.

Wang, C. & Powell, J.E. 2004. Cellulose bait improves the effectiveness of Metarhizium anisopliae as a microbial control of termites (Iso.: Rhinotermitidae). Biological Control, 30: 523-529.

Yanagawa, A., Fujiwara-Tsujii, N., Akino, T., Yoshimura, T. & Yanagawa, T. 2011. Behavioral changes in the termite, Coptotermes formosanus (Isoptera), inoculated with six fungal isolates. Journal of Invertebrate. Pathology, 107(2): 100-106.