Inhibitory effects of ethanolic and aquatic extracts of Cinclidotus fontinaloides (Hedw.) P. Beauv. on phytopathogenic fungi Fusarium solani and Bipolaris sorokiniana in vitro and in vivo

Subject Areas : Plant Pests

Roghieh Asadboland ¹ , Mohammad Reza Eslahi ² , AliReza Iranbakhsh ³ , Saeed Shirzadian ⁴

1 - PhD Student, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Assistant Professor, Department of Plant Protection, Khouzestan Agriculture and Natural Resource Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Ahvaz, Iran
3 - Professor, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
4 - Associated Professor, Department of Botany, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

Received: 2020-09-13 Accepted : 2020-09-13 Published : 2020-02-20

Keywords: Extract, Mosses, Benomyl, Difnoconazole, Tetraconazole,

Abstract :

Mosses are a group of simple, small and xerophyte plants that have been shown to have anti-cancer, anti-microbial and anti-fungal effects. In this study, the inhibitory effects of ethanolic and aquatic extracts of a moss species named Cinclidotus fontinaloides on the growth of two phytopathogenic fungi Fusarium solani and Bipolaris sorokiniana, the causal agents of root and stem rot in crops, especially wheat, were studied by using disk diffusion method on PDA medium and mixing with culture medium to determine the MIC and compared with the effect of industrial fungicides benomyl, difenoconazole and tetraconazole. Data were analyzed by using SAS software v. 9.2 and the means were compared by using Duncan's test (P <0.05). The results confirmed the inhibitory effect of different extracts on the fungi, but ethanolic extracts showed higher growth inhibitory effects on F. solani; further more aquatic extract had the same effects on tested fungi. To investigate the effect of moss extracts on the growth of these fungi in vivo, Chamran cultivar seeds were mixed with moss extracts planted in pots containing a ratio of 1:10 mixture of soil and soil contaminated with fungi and kept in a greenhouse at 25 ° C and natural light for 35 days. After the prescribed period, the rate of root and stem rot of wheat plants was examined. The results show a reduction in the rate of rot in treated pots compared to control pots.