Identification of Lavasan Soil Streptomyces and Their Antagonistic Potential

Subject Areas : Plant Pests

Seyed Hesam Tavabe Ghavami ¹ , Abul Qasem Ghasemi ² , Samin Seddigh ³

1 - Department of Plant Pathology, College of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran.
2 - Scientific staff, Iranian Research Institute of Plant Protection, Tehran, Iran
3 - Department of Plant Protection, College of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran

Received: 2015-12-23 Accepted : 2016-04-21 Published : 2016-05-21

Keywords: soil, Antibiotics, Streptomyces, antagonists,

Abstract :

Streptomyces are a group of wild-type bacteria and generally saprophytic, more than 400 species have been described and few species are pathogenic. These bacteria exist in many natural environments and antibiotic production is one of the most prominent properties. Few species have parasitic relation with animals and plants, including potato common scab disease. In this research, soil samples were gathered from fields and gardens of Lavasan region at depths of 10-30 cm. After drying and sieving, samples were diluted and cultured in casein-glycerin-agar (CGA). After a week, spored colonies with characteristics of Streptomyces were selected and transferred to the new media. The isolates were classified into three main groups based on phenytoin characteristics including color and form of spore, colony color and melanin production, consumption of hydrocarbons and antibiotic production in culture media. Some strains of each group were identified using 16sRNA sequencing and their phylogeny was investigated. In totally, 38 strains were isolated from 20 soil samples. The results showed that the strains belonged to S. kanamyceticus, S. violascens, S. akiyoshiensis and S. ambofaciens, with a similarity of more than 99%.