Etiology of leaf spot and blistering disease of cucumber and Melon in Mazandaran province

Subject Areas : Plant Pests

Minochehr Arsalani ¹ (Department of Plant Pathology, College of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran.)
Abul Qasem Ghasemi ² (Scientific staff, Iranian Research Institute of Plant Protection, Tehran, Iran)
Mojdeh Maleki ³ (Department of Plant Pathology, College of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran)

Received: 2015-10-24 Accepted : 2016-04-21 Published : 2016-05-21

Keywords: cucumber, melon, Blister spot, leaf necrosis, <Pseudomonas>,

Abstract :

Cucumber is one of the most important crops cultivated in greenhouses and field in Iran. Melon is one of members of Cucurbitaceae family and it has a large sweet fruit. Pseudomonas syringae is a bacterial plant pathogen that causes necrosis in aerial parts of the plants. As isolated P. syringae pv. lachrymans and P. viridiflava have been isolated from cucurbits in Iran and other bacteria not reported as pathogenic bacteria. Whereas agent of blister spot and leaf necrosis of cucumber andnot detected from Iran specially in Mazandaran. In this study, the cause of blisters and necrotic leaf spots cucumbers and melons after isolation from infected plants was identified by using morphological and molecular methods in Mazandaran. Based on the results of molecular tests and sequencing of the rpoB gene sequence, our bacterial agent was similar to about P. straminea, with 99 percent similarity. The results of this study showed the studied bacterium belongs to P. Straminea, and based on rpoB gene sequence closest species to it, was P. mendocina.