The Effect of Incremental Resistance Training on the Expression of Gamma Acetylcholine Receptor Subunit Genes and Semaphorin-a3 in the Gastrocnemius Muscle of Male Rats.

Subject Areas : Physical Activity and Health

Mozhgan Hassanzadeh ¹ , Mohammad Azarbayjani ^{2 *} , Shahin Riyahi Malayeri ³ , مقصود پیری ⁴ , حسن متین همائی ⁵

1 - Department of Exercise Physiology,Central Tehran Branch, Islamic Azad Univer
2 - Department of Exercise Physiology,Central Tehran Branch, Islamic Azad University, Tehran, Iran.
3 - Associate Professor of Exercise Physiology, Department of Physical Education and Sport Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran
4 - Department of Exercise Physiology,Central Tehran Branch, Islamic Azad University, Tehran, Iran.
5 - Department of Exercise Physiology,Central Tehran Branch, Islamic Azad University, Tehran, Iran.

Received: 2024-02-06 Accepted : 2024-08-21 Published : 2025-02-24

Keywords: Increasing Resistance Training, CHRNG, Semaphorine-a3, Neuro Muscular Junction,

Abstract :

Aim: Increasing the activity of the gamma acetylcholine receptor subunit and semaphorin a3 by affecting the transmission of nerve messages to the muscle causes a decrease in skeletal muscle function. The evidence shows that regular physical activities can lead to the development of muscle function by affecting the expression of these two genes. The purpose of this study was to investigate the effect of increasing resistance training on the expression of acetylcholine receptor gamma (CHRNG) and semaphorin-a3 (Sema3A) subunit genes in the gastrocnemius muscle of male rats. Method: In an experimental study, 12 six-week-old male rats, with an average weight of 195-220 grams, were randomly divided into 2 groups (6 in each group), resistance training (RT) and control (Control). The resistance training group performed incremental resistance training 5 days a week for 4 weeks. Twenty-four hours after the last training and recovery session, the subjects' sacrificial and biceps muscles were extracted to determine the expression of CHRNG and Sema3A genes by Real-time method. Results: The expression of CHRNG (P=0.044) and Semaphorine-a3 (P=0.040) genes decreased significantly in the resistance training group compared to the control group. Conclusion: The results of the present study showed that incremental resistance training can improve neuromuscular function by reducing the expression of CHRNG and Sema3A genes in the neuromuscular junction. Based on this, it is recommended to use increasing resistance exercises to improve muscle performance.

References:


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