Interactions between muscle tissue and bone metabolism in arthritic mice model following three treatment methods, exercise, ozone and stem cells.

Subject Areas : Physical Activity and Health

zahra hadadpor ¹ , Hajar Abbaszadeh ² , parvin farzanegi ³

1 - Ph.D Candidate, Exercise Physiology Department, Sari Branch, Islamic Azad University, Sari, Iran
2 - Associate Professor, Exercise Physiology Department, Sari Branch, Islamic Azad University, Sari, Iran
3 - Associate Professor, Department of Physical Education and Sports Science, Sari Branch, Islamic Azad University, Sari, Iran

Received: 2023-07-02 Accepted : 2023-08-02 Published : 2023-07-23

Keywords: stem cells, Ozone, Arthritis, Exercise,

Abstract :

Aims: Objective: a-actinin3, pgc-1α genes are involved in muscle changes related to bone problems. The aim of the present study was to determine the effect of three treatment methods, exercise, ozone and stem cells on regulatory and inhibitory factors affecting the interactions between muscle tissue and bone metabolism in arthritic mice model.Materials and methods: 63 male mice (average age 10 weeks and average weight 250 to 300 grams) into 9 groups; Healthy control, control-patient, patient-saline, patient-exercise, patient-ozone, patient-cell, patient-cell-ozone, patient-exercise-ozone and patient-exercise-cell were divided. Osteoarthritis was induced to mice by surgical method. The training program included 30 minutes of running on the treadmill without a slope at a speed of 16 meters per minute. 1 x 106 cells/kg were injected into mice. Ozone was injected in the tifofemoral joint line of the knee with a concentration of 20 µg/ml. The expression of α-actinin3, pgc-1α gene was measured by real time PCR method.Findings: cell+exercise, exercise+ozone, and cell+ozone groups caused a significant increase in the expression of a-actinin3, pgc-1α genes in the thigh muscle compared to the cell, exercise, and ozone groups (P<0.05). ). In the exercise+cell group, these changes were more evident.Conclusion: The results showed that a combination of exercise and cells with an increase in the expression of a-actinin3, pgc-1α genes of the thigh muscle can probably have beneficial effects on the interactions between muscle and bone in the arthritic rat model, and as a result, the risks of analysis and Reduce muscle weakness caused by the complications of arthritis.