Investigating of malondialdehyde levels in azoospermia model rats following a period of swimming exercises and stem cell transplantation

Subject Areas : Physical Activity and Health

sahar kochaki ¹ , Hajar Abbaszadeh ² , parvin farzanegi ³

1 - Ph.D Candidate, Exercise Physiology Department, Sari Branch, Islamic Azad University, Sari, Iran
2 - Associate Professor, Exercise Physiology Department, Sari Branch, Islamic Azad University, Sari, Iran
3 - Associate Professor, Exercise Physiology Department, Sari Branch, Islamic Azad University, Sari, Iran

Received: 2023-01-30 Accepted : 2023-02-01 Published : 2022-12-22

Keywords: Malondialdehyde, azoospermia, Exercise,

Abstract :

The purpose of this study was to investigate the malondialdehyde levels in azoospermia model rats following a period of swimming training and stem cell transplantation. Thirty 8-week-old rats were selected, and then the azoospermia model was created with the drug busulfan with a dose of 40 mg. Rats were randomly divided into healthy control (n=5), sham control (n=5), azoospermia control(n=5), azoospermia+exercise (n=5), azoospermia+cell (n=5) and azoospermia+cell+exercise (n=5) groups. One month after the creation of the one-time model, stem cells were transplanted in the vas deferens in the amount of one million cells for each mouse, and after recovery, daily for 30 minutes in They swam 5 days a week for 8 weeks. Malondialdehyde levels were measured by thiobarbituric acid method and all calculations were done using SPSS/23 statistical software and at a significant level of p≤0.05. The results showed that induction of azoospermia model increased testicular tissue malondialdehyde levels (P=0.002) and exercise combined with cell therapy caused a non-significant decrease in testicular malondialdehyde levels in azoospermia model rats (P>0.05). In general, the results of the present research indicate an increase in malondialdehyde levels in testicular tissue cells of azoospermia model mice. On the other hand, regular aerobic exercise such as swimming along with cell therapy will probably help in controlling the effects of infertility diseases by reducing malondialdehyde levels and reducing oxidative stress.