Effect of High Intensity Interval Training on MiR-193b and Insulin Resistance Index in Diabetic Rats
Subject Areas : New studies in exercise metabolism and physical activity
Masoud Alizadeh
1
,
Najmeh Rezaeian
2
1 - PhD Student in exercise physiology. Department of physical education, Bojnourd branch, Islamic Azad University, Bojnourd, Iran.
2 - Department of physical education and sport science, Bojnourd branch, Islamic Azad University, Bojnourd, Iran
Keywords: MiR-193b, High Intensity Interval Training, Diabetes, Insulin Resistance, Obesity,
Abstract :
miRNA are emerging as important regulators of key biological processes and involved in the pathogenesis of various diseases such as Type 2 diabetes. Therefore, the Purpose of this study was to investigate the effect of six weeks of high intensity interval training (HIIT) on adipose tissue levels of MiR-193b and insulin resistance index in male diabetic rats with high fat diet and streptozotocin. Twenty male wistar rat (10-12 weeks old, 370/25±13/76 gr) selected and following the induction of type 2 diabetes, the diabetic rats were randomly divided into experimental and control groups. The rats in the experimental group participated in six weeks of high intensity interval training of running on a treadmill with 40 seconds of repetitions with 2 seconds of active rest between each repetition, 30 minutes per session and five sessions per week. All rats were dissected 48 hours after the last training session and the blood indices were evaluated using appropriate laboratory methods. Data analysis were done using independent and paired t-test and Pearson's correlation test at a significance level of less than 0.05. Results: Adipose tissue levels of MiR-193b following six weeks of HIIT in experimental group were higher than control group; whereas, levels of insulin and fasting blood glucose , insulin resistance index and body weight were in the experimental group compared to the control group. Furthermore, Six weeks of high intensity interval training in experimental group caused in significant decreases in body weight in post- test compared to pre- test . Moreover, according to the Pearson correlation test, there were negative significant correlations between the changes in levels of adipose tissue levels of MiR-193b following HIIT and changes in body weight (P=0.038). Six weeks of HIIT play role in improving insulin resistance by increasing the MiR-193b levels in addition to improve body composition.