The effect of incremental aerobic exercise after injection of bone marrow-derived stem cells on some indicators of oxidative stress and inflammatory of liver tissue in rats model of fatty liver

Subject Areas : New studies in exercise metabolism and physical activity

hamed Namdar ¹ , Seyed Abdollah Hashemvarzi ² , Abdorreza Jafari Chashmi ³

1 - Ph.D Candidate in Exercise Physiology, Department of Exercise Physiology, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran
2 - Exercise Physiology Department, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran
3 - Exercise Physiology Department, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran

Received: 2023-12-10 Accepted : 2024-01-30 Published : 2023-11-22

Keywords: Exercise, Inflammation, Oxidative stress, Fatty liver, Stem cell,

Abstract :

Introduction: Non-alcoholic fatty liver disease is one of the most common liver diseases of this century, which is associated with changes in inflammatory cytokines and increased oxidative stress.The purpose of this research was to investigate the effect of an increasing aerobic exercise period after bone marrow stem cell injection on some indicators of oxidative and inflammatory stress of liver tissue in rats model of fatty liver. Methods: 60 adult male Wistar rats were divided into two main groups: control (6 rats) and patients with fatty liver (54 rats). The control group used standard rodent food for 6 weeks, while the animals in the diseased groups used high-fat food to induce fatty liver disease. At the end of the sixth week, to confirm the development of fatty liver disease, blood was randomly drawn from the tails of 3 mice and ALT and AST enzymes were measured. Then the animals of the patient group were randomly divided into 5 groups: Saline, Fatty liver, Exercise, Cell, Exercise + Cell. About 105 cells in 2 microliters of medium were injected into the cell receiving groups for each rat through the tail vein. The training program consisted of 8 weeks of incremental treadmill running.Results: The levels of CAT and SOD increased in the treated groups, but the level of the inflammatory index TNF-α decreased, but these changes were significant only in the groups of exercise and exercise + cells compared to the fatty liver group.Conclusion: Incremental aerobic training after the injection of bone marrow-derived stem cells increased the antioxidant levels of CAT and SOD and decreased the inflammatory index of TNF-α in mice with fatty liver. Therefore, it seems that this method can improve liver condition and be considered as a therapeutic solution.