Isolation of β-1 and 3-Gloconase from Medicago truncatula, exspress them in E.coli BL21 and investigation of their fungicide
Subject Areas : New Finding in AgricultureN. Nabavi 1 , B. Najmi 2 , M. Rasooli 3
1 - M. Sc.Student in Agricultural Biotechnology
2 - M. Sc. in Agronomy and Plant Breeding
3 - Ph. D. Student in Plant Breeding, Islamic Azad University, Sciense and Research Branch, Tehran, Iran
Keywords: β-1and 3-Glucanase, Medicago truncatula, Pathogenesis related proteins,
Abstract :
Pathogenesis related proteins are compounds that produced by response to pathogens or environmental stress. Up to now numerous kind of β-1 and 3-Glucanase are identified in various plants and thein fungicide effect were tested. The aim of this research was isolation, over-expression and analysis of physiological activities of β-1 and 3-Glucanase (PR-2) from Medicago truncatula. In this case by Medicago sativa and Pisum sativum β-1 and 3-Glucanase gene alignment on Medicago truncatula genome three open reading frame with %50 similarity by having start and stop codon were identified and isolated that fragments were cloned in PET21c vector and expressed in E.coli BL21. SDS PAGE technique reveal that proteins were produce as an inclusion bodies. We also test their fungicide effect after folding them to achieve their natural structure on Alternaria alternaria and Fusarium graminearum. The result were significant ( P< 0.05) by SAS system analysis.
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