Detection of Bacterial Vaginosis Associated with Gardnerella vaginalis Using Molecular Methods in Women Attending Healthcare Centers

Subject Areas : microbiology

Parisa Motamedian ^{1 *} , Kimia Golestanfar ² , fatemeh Khodaverdipour ³

1 - 1. Department of Microbiology, ShK.C., Islamic Azad University, ShahreKord, Iran
2 - Department of Microbiology, ShK.C., Islamic Azad University, ShahreKord, Iran
3 - Department of Microbiology, ShK.C., Islamic Azad University, ShahreKord, Iran

Received: 2025-06-30 Accepted : 2025-07-11 Published : 2025-07-11

Keywords: Gardnerella vaginalis, Bacterial Vaginosis, Molecular Method,

Abstract :

Genital tract infections are among the most common reasons for women to seek medical care. Gardnerella vaginalis is a predominant pathogen in bacterial vaginosis and plays a significant role in producing symptoms such as malodorous vaginal discharge and inflammatory complications. This infection is associated with an increased risk of upper genital tract diseases, including endometritis after cesarean section or abortion, surgical site infections, pelvic inflammatory disease, preterm labor, chorioamnionitis, and reduced success rates in assisted reproductive technologies. Rapid and accurate diagnosis not only facilitates timely treatment but also helps prevent the spread of infection. In this study, 250 samples were collected from women presenting with vaginitis symptoms at clinics in Shahrekord. The samples were analyzed using macroscopic, microscopic, biochemical, and molecular (PCR) methods. Results revealed that 220 out of 250 samples (88%) were positive for Gardnerella vaginalis infection. These findings highlight the high prevalence of Gardnerella vaginalis among symptomatic women and emphasize the importance of using accurate diagnostic tools for effective management and prevention of bacterial vaginosis

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