Molecular characterization of Staphylococcus aureus strains isolated from wound and urine in Kermanshah, Iran.
Subject Areas : microbiologyzeinab ahmadi 1 * , hossein Aghajani 2 , Hossein Khodabandeh shahraki 3
1 - 1. Department of Microbiology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2 - 1. Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
3 -
Keywords: Staphylococcus aureus, antibiotic resistance patterns, multiplex-PCR, RFLP-PCR, coagulase gene,
Abstract :
Human is the main source of Staphylococcus aureus in the nature. This bacterium is one of the important agents of nosocomial infections. Therefore, is study molecular characterization of this bacteria of high important. After identification and confirmation of 100 strains of Staphylococcus aureus isolated from wounds and urine samples in Kermanshah with microbiology standard methods, the antibiotic susceptibility testing was done and resistance genes (mecA, aacA-D, tet K, tet M, msrA, ermA) of isolates were studied with multiplex-PCR method and genotyping of coagulase gene of isolates was studied by RFLP-PCR method. In susceptibility testing were reported greatest resistance to penicillin (90%) and lowest resistance nitrofurantoin (8%). In multiplex-PCR method, (49%) of the isolates carry mecA gene, (24%) aacA-D, (89%) tetM, (14%) tetK, (40%) ermA and (36%) msrA. In the detection polymorphism of coagulase gene from 65 strains of Staphylococcus aureus isolated from urine, coagulase gene polymorphism was observed in 53 cases, genotype I (61.53%) and genotype VIII (12.3%) and genotype IX (7.69%). The coagulase gene polymorphism of strains isolated from wound were observed in 22 samples. Genotype I was reported (42.85%) and genotype VIII (20%) was observed. Detection molecular characterization of isolates is very effective and efficient in prevention and control infection.
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